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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2018 Mar 28;518:358–368. doi: 10.1016/j.virol.2018.03.011

Figure 7.

Figure 7.

Comparison of HBsAg secretion efficiency among 26 immune escape or glycosylation mutants. Huh7 cells grown in 6-well plates were transfected with 2.0 μg of the 0.7mer L+/M+/S+ construct. Both cells and culture supernatant were harvested 5 days later. HBsAg was measured from 20 ul of 1:1000 diluted cell lysate, and 25 ul of 1:100 diluted culture supernatant, using an ELISA kit from KHB. The experiments were repeated four times. (A) HBsAg (OD450) values in cell lysate. (B) HBsAg (OD450) values in culture supernatant. (C) Ratio of extracellular HBsAg/intracellular HBsAg, with value for the WT virus set arbitrarily at 1.