Figure 6. Modulation of cytokine profiles in an in vitro model of intestinal inflammation by metabolites from SHIME reactors treated with the probiotic with digestive enzymes supplement.

Samples of SHIME suspensions were taken from reactors with curative (CUR) and preventive (PREV) administration of the probiotic with digestive enzymes supplement as compared to a control SHIME (CTRL; no supplement) from the control period (baseline microbial community prior to 5-FU/vancomycin) and treatment period with 5-FU/vancomycin. Cytokine levels of IL-6 (A) and IL-10 (B) were measured 6 h after LPS treatment on the basolateral side of the Caco2/THP-1 co-cultures after pre-treatment of the apical side for 24 h with the SHIME samples. The red dotted line corresponds to the experimental control consisting of LPS. Data are plotted as the mean ± SEM. The chart (lower panel) outlines the corresponding statistical analyses performed using two-way ANOVA with Dunnett's multiple comparisons test. Significance is depicted where (^*). (^*), (^**), (^***) and (^****) represent p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively. Ns = non-specific; PC: proximal colon; DC: distal colon.