Skip to main content
. 2018 Aug 14;7:e35314. doi: 10.7554/eLife.35314

Figure 3. DFT2 tumours express variable levels of classical MHC class I (Saha-UA, -UB and -UC) and non-classical MHC class I (Saha-UK) in vivo.

IHC staining of DFT2 tumours (DFT2_RVT1, DFT2_SNT2, DFT2_818T1, DFT2_547T1, DFT2_523 DFT2_812) with α-UA/UB/UC_15-25-18 against Saha-UA, -UB and -UC and α-UK_15-29-1 against Saha-UK. Arrows indicate tumour cells for each biopsy; arrow heads indicate stroma separating nests of tumour cells where present. Isotype and secondary antibody controls can be found in Figure 3—figure supplement 2. Boxed insets are at 600 x magnification and are taken from areas indicated by arrows. Scale bars represent 50 μm. Positive cells for each marker are stained brown, nuclei are stained blue.

Figure 3—source data 1. Nucleotide sequences for the pET22B + SahaUC and pET22B + SahaUK constructs used to test the specificity of the MHC class I antibodies (a- UA/UB/UC 15-25-18 and a- UK 15-29-1) described in Figure 3.
elife-35314-fig3-data1.txt (2.1KB, txt)
DOI: 10.7554/eLife.35314.010

Figure 3.

Figure 3—figure supplement 1. Western blots illustrating specificity of the MHC class I heavy-chain monoclonal antibodies.

Figure 3—figure supplement 1.

Western blot on classical MHC class I protein (Saha-UC) (38 kDa) and non-classical MHC class I protein (Saha-UK) (39 kDa) with MHC class I heavy-chain specific antibodies.
Figure 3—figure supplement 2. Isotype controls (IgG1b – anti-SahaUA/UB/UC and IgG2b – anti-SahaUK) and secondary antibody only controls for the immunohistochemistry presented in Figure 3.

Figure 3—figure supplement 2.

All images are 100 x magnification with scale bars represent 100 μm.
Figure 3—figure supplement 3. DFT2 tumours have different growth patterns.

Figure 3—figure supplement 3.

H and E staining of four DFT2 tumours representing different growth patterns. Scale bars represent 100 μm.