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Increased expression of aadB in cells with increased tobramycin resistance. Expression of aminoglycoside resistance genes aadB (A) and aacA4 (B) was quantified by qRT-PCR. Data are presented as the averages and standard errors of means of three independent biological replicates (*, P < 0.05, and ****, P < 0.0001, relative to AB5075 VIR-O by one-way analysis of variance with Dunnett’s posttest).
