TABLE 3 .
aadB copy number in the presence and absence of antibiotic selection
| Strain | Copy no. (mean ± SD) of genea: |
|
|---|---|---|
| aadB | ABUW_RS19335 | |
| hetR-O2 + Tob | 40.35 ± 0.62 | 0.72 ± 0.16 |
| hetR-O2 − Tob | 16.75 ± 0.16* | 0.71 ± 0.03 |
| hetR-T1 + Tob | 55.12 ± 4.35 | 0.72 ± 0.05 |
| hetR-T1 − Tob | 40.38 ± 10.71 | 0.77 ± 0.08 |
a
Copy numbers of aadB and ABUW_RS19335 relative to aacA4 in hetR-O2 and hetR-T1 colonies grown with (+) or without (−) tobramycin for 24 h. The means and standard deviations of two biological replicates are shown. The copy number of aadB was significantly decreased in hetR-O2 grown without tobramycin compared to this strain grown in the presence of drug (*, P < 0.05 by paired two-tailed t test).