Irreversible electroporation of malignant liver tumors: Effect on laboratory values

Abstract

Liver cancer is often associated with chronic liver diseases. Treatment with percutaneous irreversible electroporation (IRE) may preserve liver function. In the present study, the clinical data of 29 patients with liver tumors between July 2015 and December 2016, all of whom underwent liver IRE at Fuda Cancer Hospital, Guangzhou, China was retrospectively reviewed. All the patients survived the treatment. Of the 29 patients, 7 were positive for hepatitis B, 15 had hepatocellular carcinoma (HCC) and 7 had pancreatic cancer with liver metastases. All patients survived IRE. Despite liver-protective treatment prior to IRE, the mean alanine transaminase (ALT) and aspartate transaminase (AST) levels were significantly elevated 1–2 days after IRE, to 540 and 712 U/l, respectively; however they had returned to the preoperative values by 2 weeks following IRE. Prior to IRE, the mean total bilirubin and direct bilirubin measurement levels were normal; however, 8–10 days after IRE, they had increased to 24 U/l and 12 µmol/l, respectively, and had returned back to the preoperative levels by 2 weeks after IRE. This first group included all patients. The result of the 4 subgroups of cancer patients demonstrated a variation between different measurement days and recovery with patients positive for the hepatitis B virus taking the longest duration to recover (17±3 days) meanwhile patients with pancreatic cancer with liver metastases took the shortest time to achieve recovery (10.78±2 days). The findings of the present study indicate that hepatic injury caused by IRE is transient and self-limiting in patients with liver tumors.

Introduction

Hepatocellular carcinoma (HCC) is a common tumor worldwide and its incidence rate is increasing (1). Of the affected patients, 80% have chronic liver diseases such as hepatitis or cirrhosis; the presence of these conditions in conjunction with HCC has a major effect on the prognosis and treatment of this type of cancer (2) Currently, percutaneous ablation therapies, including percutaneous ethanol injection, microwave coagulation and radiofrequency ablation, are available and are important therapeutic modalities for the treatment of HCC (3–8). The hepatitis B virus (HBV) is responsible for 30% of all cases of cirrhosis and >50% of HCC cases with an incident rate of 50 million new cases diagnosed annually in endemic countries (9). Irreversible electroporation (IRE) is a newly developed non-thermal ablation procedure where electrical pulses are delivered for a few milliseconds to induce nanoscale defects that increase cell membrane permeability, the procedure induces apoptosis without harming the extracellular matrix; therefore, the structural components of the tissues are preserved (10,11). IRE is a novel, non-thermal form of tumor ablation that is not affected by heat sink and may result in less collateral damage based on its mechanism of action (12). IRE relies on short pulses of high-frequency energy to induce pores in the lipid bilayer of cells, leading to cell death via apoptosis. In the present study, hepatic injury in 29 patients following a single IRE session was retrospectively assessed. The serum transaminases and bilirubin values were compared between patients who were positive and those who were negative for hepatitis B and for patients with HCC and pancreas cancer with liver Metatases, to study the effects of IRE on liver function and its recovery.

Materials and methods

Ethics

The present study was approved by the Regional Ethics Committee of Guangzhou Fuda Cancer Hospital (Guangzhou China). Written informed consent was obtained from all patients, and the study protocols were in accordance with the tenets of the Declaration of Helsinki.

Patient selection

Diagnosis of unresectable HCC and liver metastasis was confirmed by pathological studies. The diagnoses were confirmed blindly by two pathologists at pathology department affiliated with Jinan University (Guangzhou, China) and imaging analyses and measurement of the tumor marker α-fetoprotein. Patients who met the following criteria were considered to be eligible for the study: The presence of one or two significant tumors in the liver, Karnofsky performance status (13) (KPS) score ≥70, white blood cell count ≥3×10⁹/l, neutrophil count ≥2×10⁹/l; hemoglobin ≥90 g/l, platelet count ≥100×10⁹/l, prothrombin time (international normalized ratio, INR) ≥1.5. Furthermore, the present study excluded patients who had severe coronary heart disease, myelosuppression, respiratory disease, acute/chronic infection, or level 3 hypertension, and had adequate hepatic function [total bilirubin (T.BIL) <75 µmol/l, direct bilirubin (D.BIL) <39 µmol/l, and a Child-Pugh score (14) of A or B] and renal function (serum creatinine <130 µm and serum urea <10 mm). Between July 2015 and December 2016, 29 patients (20 males; age range, 32–75 years; mean age, 55 years; and 9 females; age range, 32–70 years; mean age, 53 years) were eligible and enrolled from Fuda cancer hospital, Affiliated with Jinan University (Guangzhou, China).

Irreversible electroporation procedure

All patients underwent gastric decompression and endotracheal intubation. General anesthesia was induced intravenous and inhalation anesthesia by trachea intubation, drugs delivered via vein and trachea by intravenous infusion, including midazolam (0.5 mg) and penehyclidine hydrochloride (0.5 mg). For general anesthesia induction: Etomidate (0.3 mg/kg), benzenesulfonic acid cisatracurium (0.2 mg/kg) and remifentanil (150 µg) were used, and for maintenance: Intravenous pumping of diprivan (50–150 mg/h); remifentanil (320–720 µg/h) and inhalation of sevoflurane (0.6–2%) benzenesulfonic acid cisatracurium (6 µg/kg). Computed tomography (CT) was undertaken and the target region was demarcated. Ultrasound-guided insertion of 2–3 electrodes was performed. Following anesthesia an additional CT image was taken to confirm correct placement of the electrodes. IRE was synchronized to deliver electrical pulses coordinated with cardiac rhythm to prevent cardiac dysrhythmia. Typically, the distance between electrodes was 1.5–2 cm. The voltage was set at 1,500–3,000 kV. Between 70–90 pulses were delivered in 7–9 sets of 10 pulses. Additional pullbacks were performed if the target region was >2 cm in diameter. The baseline and highest heart rate during IRE were recorded by electrocardiography. Arrhythmia of any form was documented. Invasive blood pressure measurement via the femoral artery was applied for precise systolic blood pressure (SBP) monitoring. If SBP was >40 mmHg during ablation or >190 mmHg at any time, electric pulses were suspended for 2–3 min. If no obvious decrease in SBP was observed after 2–3 min, 2–5 mg of phentolamine was administered intravenously to lower blood pressure and prevent hypersensivity.

Assessment of hepatic functional reserve

Hepatic function was assessed with an automatic biochemical analyzer (7100; Hitachi Ltd., Tokyo, Japan). The alanine transaminase (ALT) and aspartate transaminase (AST) levels were measured with the velocity method using a specialized reagent kit (AST kit: Aspartate Aminotransferase kit [Aspartate Substrate Method, cat. no. 1740-2013. ALT kit: Alanine aminotransferase kit. (Alanine low content method) no. 170781], and the T.BIL and D.BIL levels were measured using the vanadate method with a commercially available kit [TBIL: Total Bilirubin kit (Vanadate Oxidation Method) no. 1737-2013. D.Bil: Direct Bilirubin kit (Vanadate Oxidation Method) no. 4523-40-201] (BioSino Bio-Technology and Science Inc., Beijing, China). Blood samples were obtained in the morning, after overnight fasting. The tests were performed every 1–3 days, until the patients were discharged (on day 20). The normal ranges for the measured parameters were as follows: ALT, 5–35 U/l; AST, 8–40 U/l; T.BIL, 0–25.5 µmol/l; and D.BIL, 0–13 µmol/l. Values that were above the upper limit of the normal range were considered to indicate abnormal hepatic function.

Statistical analysis

The revised version of the Response Evaluation Criteria in Solid Tumors (version 1.1) (15) was used to determine the response of the hepatic tumors to the treatment. Statistical tests were performed with commonly used methods, and a nonparametric, one-way AVONA followed by Bonferroni's multiple comparison post-hoc test was used for comparisons between days. Test results are expressed as the mean ± standard error and P<0.05 was considered to indicate a statistically significant difference. All analyses were conducted using GraphPad software version 5.0 (GraphPad, Software, Inc., La Jolla, CA, USA).

Results

Clinical data

Prior to hepatic IRE, detailed data of the patients were extracted and are presented in Table I. Of the 29 patients, 22 (75.9%) had HCC, 7 (24.1%) had a history of hepatitis, 7 (24.1%) had pancreatic cancer with liver metastases, 10 (34.5%) had undergone initial surgical treatment and 22 (75.86%) had undergone systemic chemotherapy at different centers.

Table I.

Detailed data on patients prior to hepatic irreversible electroporation.

Characteristic	n (%)
Sex
Female	9 (31.03)
Male	20 (69.97)
HCC	22 (75.9)
Sex (male/female)	15/7
Median age (years)	52
AJCC stage (2010) (37,38)
IIIA	2 (9.09)
IIIB	1 (4.55)
IIIC	2 (9.09)
IVA	17 (77.27)
Hepatitis B positive	7 (24.1)
Child-Pugh stage (15)
A	14 (63.64)
B	8 (36.36)
Liver function
ALT, U/l	27±11
AST, U/l	44±43
T.BIL, µmol/l	18±7
D.BIL, µmol/l	7±4
Tumor type
Single massive	17 (77.27)
Multinodular	5 (22.73)
Ascites
Yes	14 (63.64)
No	8 (36.36)
Pancreatic cancer with liver metastasis	7 (24.1)
Sex (male/female)	5/2
Median age (years)	53
AJCC stage (24,38)
III	0
IV	7
Liver function
ALT, U/l	35±11
AST, U/l	49±23
T.BIL, µmol/l	14±4
D.BIL, µmol/l	7±4

AJCC, American Joint Committee on Cancer staging system; HCC, hepatocellular carcinoma; ALT, alanine aminotransferase; AST aspartate aminotransferase; T.BIL, total bilirubin; D.BIL, direct bilirubin.

Perioperative outcomes

All the hepatic lesions were treated with IRE, which was performed successfully in all patients. Severe complications (such as rupture or hepatic failure, myoglobinuria and acute renal failure) did not occur following IRE. There were several mild adverse effects [7 patients developed fever (38.2–39.8°C), 15 patients had pain for 3–7 days, 1 patient had infection, 2 patients had abdominal ascites, and 6 patients developed variable degree of nausea and vomiting]; however the patients recovered without symptomatic management.

Changes in hepatic functional reserve after irreversible electroporation

Of the 29 patients, 7 were positive for the hepatitis B virus, 15 had HCC), and 7 had pancreatic cancer with liver metastases. A total of 29 IRE procedures were performed. The transaminase levels were abnormal until the 4th session of ablation, and the bilirubin level was abnormal until the 2nd session of ablation (this was the case for all patients). The serum transaminase levels increased rapidly and reached a peak on day 1 following IRE, after which they gradually decreased. The ALT and AST levels demonstrated a significant decrease from day 5 and 7, respectively (P<0.001; Fig. 1A and B). No significant change in the serum bilirubin level was observed until 20 days after IRE (Fig. 1C and D).

Figure 1.

Variations in hepatic functional reserve after irreversible electroporation in the 29 patients of the present study. Bonferroni's multiple comparison was used for comparison of values between the stated time points. The number of results varied as follows: 22, 18, 18, 16, 10, 15, 8, 9, 6, 8 and 4: 22 results were obtained on days 0, 1–2, 3–4, 5–6, 7–8, 9–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The markers of hepatic functional reserve were (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. ***P<0.001 vs. IRE, irreversible electroporation; ALT, alanine transaminase; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.

Changes in hepatic functional reserve in seven patients with hepatitis B

Each of the 7 patients who were positive for hepatitis B underwent IRE. Prior to the 2nd and 3rd ablation sessions, the transaminase and bilirubin levels were abnormal (data not shown). Additionally, 1 day after IRE was performed, the serum transaminase levels increased rapidly until they reached a peak (P<0.001), after which they gradually decreased. The ALT and AST levels showed a significant decrease from day 5 (P<0.01) and day 3, respectively (P<0.001; Fig. 2A and B). There was no obvious variation in the serum bilirubin level until 20 days following IRE (Fig. 2C and D).

Figure 2.

Variations in hepatic functional reserve in seven patients with hepatitis B. Bonferroni's multiple comparison post-hoc test was used to analyze differences in values between time points. The number of results obtained varied as follows: 18, 8, 6, 4, 4, 5 and 6: 6 results were obtained on days 0, 1–2, 3–4, 5–6, 7–8, 9–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The following markers of hepatic functional reserve were used: (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. ***P<0.001, **P<0.01 and *P<0.05 vs. Day 0, 1, 3 or 5. IRE, irreversible electroporation; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.

Changes in hepatic functional reserve in 22 patients without hepatitis B

All the 22 patients who were negative for hepatitis B underwent liver IRE. None of the patients had normal transaminase levels; in addition, the bilirubin level was abnormal before the 8th ablation session. The serum ALT and AST level increased within 2 days after IRE (P<0.01; Fig. 3A and B) and then decreased gradually, a markedly significant decrease was noted after 8–10 days. No significant change in the serum bilirubin level was observed (Fig. 3C and D).

Figure 3.

Variations in hepatic functional reserve in 22 patients without hepatitis B. Bonferroni's multiple comparison was used to compare values across time points. The number of results varied between days: 48, 16, 12, 10, 6, 11, 10 and 12: 18 results were obtained on days 0, 1, 2, 3, 4, 5, 6–7, 8–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The hepatic functional reserve markers were (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. **P<0.01 and ***P<0.001 vs. Day 0, 1 or 3. IRE, irreversible electroporation; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.

Variations in hepatic functional reserve patients with HCC

In the 15 patients with HCC who underwent IRE, the serum transaminase levels increased rapidly till they reached a peak the day after IRE, following which they decreased gradually. The serum ALT and AST levels showed a significant decrease from day 0, 1, 3 and 5, respectively (P<0.01; Fig. 4A and B). However, no significant change was observed in the serum bilirubin levels until 20 days following IRE (Fig. 4C and D).

Figure 4.

Variations in hepatic functional reserve after irreversible electroporation in 15 patients with hepatocellular carcinoma. Bonferroni's multiple comparison was used to compare values between different time points. The number of results obtained varied across time points as follows: 20, 16 16, 16, 15, 8, 9, 6 and 4: 22 results were obtained on Days 0, 1–2, 3–4, 5–6, 7–8, 9–10 and 11–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The hepatic functional reserve markers used were (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. ***P<0.001 and **P<0.01 vs. Day 0, 1 or 3 IRE, irreversible electroporation; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.

Changes in hepatic functional reserve in patients with pancreatic cancer with liver metastases

The 7 patients with pancreatic cancer with liver metastases also underwent liver IRE. The serum ALT and AST value increased within 2 days following IRE (P<0.01) and then fell gradually, with a significant decrease observed after day 10 (Fig. 5A and B; P<0.05). The serum bilirubin levels increased gradually until they reached a peak at 10–12 days after IRE (Fig. 5C and D; P<0.05), following which it decreased gradually.

Figure 5.

Variations in hepatic functional reserve in 7 patients with pancreatic cancer with liver metastases. Bonferroni's multiple comparison was used to compare values between different time points. The number of test results varied between different measurement days as follows: 18, 8, 6, 4, 4, 5 and 6: 6 results were obtained on days 0, 1–2, 3, 4, 5, 6, 7–9 and 10–20, respectively. Days on which <3 test results were obtained were merged with adjacent days. The following markers of hepatic functional reserve were used: (A) alanine transaminase, (B) aspartate transaminase, (C) direct bilirubin and (D) total bilirubin. *P<0.05, **P<0.01 vs. Day 0, 1, 3 or 10. IRE, irreversible electroporation; ALT, aspartate transaminase; D.BIL, direct bilirubin; T.BIL, total bilirubin.

Changes in hepatic functional reserve in the 4 patient subgroups

Each result of the 4 subgroups of patients with cancer demonstrated a variation between different measurement days and recovery with patients positive for the hepatitis B virus taking the longest duration to recover (recovery was considered to be when liver functions returned to normal) i.e., 17±3 days, meanwhile, the patients negative for the hepatitis B viruses and patients with HCC whose recovery is observed in 15±2.9 and 12.62±4.3 days respectively, by contrast patients with pancreatic cancer with liver metastases took the shortest time to achieve recovery 10.78±2 days. The result was statistically significant between pancreatic cancer with liver metastases and the other groups (HCC, HBV⁺ and HBV⁻; P<0.05; Fig. 6). Overall patients suffering from primary hepatic diseases took longer duration to recover when compared with patients with metastatic disease.

Figure 6.

Variations in hepatic functional reserve in the 4 patient subgroups. The patients with pancreatic cancer with liver metastases was the first group to recover to normal liver function, while the HBV+ group was the last. *P<0.05. IRE, irreversible electroporation; PA Ca; HCC, hepatocellular carcinoma; HBV+, Hepatitis B positive; HBV-, Hepatitis B negative.

Discussion

IRE is a promising procedure for unresectable hepatocellular carcinoma (16). The complications of IRE when used to treat malignant hepatic tumors (such as fever, local pain, abdominal distension, ascites, nausea and vomiting) are acceptable and there is evidence to indicate that the therapy may improve survival. Thus, it is an effective liver tumor ablative therapy that results in only mild and transient adverse effects (17–20). IRE is a novel ablation technique wherein electroporation or electro-permeabilization is used to generate electric pulses that create nanoscale defects in the cell membrane and alter its permeability (21). Ablative treatment has been identified to be safe and effective in patients with primary and secondary liver cancer, and those who are not suitable for surgery (22). In the present study, one session of IRE was performed for primary liver tumors and secondary metastases to avoid serious adverse effects. Follow-up liver function tests and routine blood tests revealed immediate liver function damage followed by recovery, consistent with the findings of a study by Chen et al (23). IRE causes significant abnormalities in liver function; however, in the majority of patients these are self-limiting, do not preclude treatment and resemble the changes observed following radiofrequency ablation or cryoablation of the liver (24).

IRE for the treatment of liver cancer is associated with hepatocyte destruction and the subsequent release of transaminases and bilirubin, which are considered as important markers of hepatic functional reserve (25). Similarly, a recent study has reported marked elevations in AST, ALT, T.BIL, and D.BIL (26), which are usually transient and normalize within a few days. It was identified that 1 day after ablation, the ALT level was 3.3-fold higher (mean peak level, 241 U/l) and the AST level was 5-fold higher (mean peak level, 427 U/l); however, they had almost reverted to the preoperative levels within 5 days following the procedure (26). A similar study (22,27) identified 15 bile duct injuries (narrowing, n=8; dilation, n=7) in subacute follow-up magnetic resonance images examinations of 3 patients demonstrated transient abnormalities of laboratory values of bilirubin, 1.6–5.2 mg/dl. Short-term laboratory values were abnormal in 1 patient (increased alkaline phosphatase of 533 U/l vs. baseline) as a result of local tumor recurrence.

Recently, Silk et al (28) reported where IRE was used to treat 22 hepatic metastases in 11 patients; the laboratory values were abnormal after 4 treatment sessions in 3 patients (bilirubin, 2.6–17.6 mg/dl; alkaline phosphatase, 130–1,035 U/l). These abnormal values were transient after 2 sessions, with 2 patients exhibiting prolonged elevation of these abnormal values and 1 requiring stent placement. In the former 2 patients, these adverse effects appeared to be secondary to tumor progression rather than bile duct injury (28). Conversely, the present study did not identify a prolonged significant increase in serum bilirubin. The participants were classified into the hepatitis B virus-positive and hepatitis B-negative groups, and an increase in T.BIL by >3 times [74 µmol/l following IRE vs. 23 µmol/l prior to IRE (3.2 times greater)] and a similar increase in D.BIL [45 µmol/l after IRE vs. 12 µmol/l prior to IRE (3.8 times greater)] were only observed in the hepatitis B-positive group at 3–5 days following treatment. The increase in AST and ALT levels observed in the present study was consistent with previous studies; however the range of increase varied between studies (18,29–31). The minimal increase that was observed in the present study may be explained by the time points of the measurements. Overall, the results indicate the importance of ALT and AST as markers of post-IRE liver function.

In patients with hepatitis B, serum bilirubin increased >3 times compared with the preoperative level 7–9 days following IRE. The reason for this increase remains unclear and warrants further research. In the present study, all the patients tolerated the treatment and there were no records of severe complications following IRE. Compared with radiofrequency ablation, IRE resulted in faster liver regeneration, which demonstrated the safety and efficacy of the technique (32). A recent prospective study of IRE treatment of 44 patients with HCC, colorectal, and other lesions reported the following local, recurrence-free survival rates: 97.4% at 3 months, 94.6% at 6 months and 59.5% at 12 months, the recurrence rates tended to be higher in lesions with diameter >4 cm (32). In a smaller cohort of 28 patients treated with IRE, portal vein thrombosis was reported in only 1 case in which the tumor was located within 1 cm of a major portal pedicle (33). Based on the findings, the authors of the study concluded that IRE was safe even for the treatment of liver lesions present near major vascular structures, and their findings are consistent with the reported theoretical benefits of non-thermal ablation (34–36). Cannon et al (32) reported that in a cohort of 44 patients, who underwent IRE for primary and secondary liver cancer, the incidence of complications was low. Only 5 patients were observed to have developed adverse events, but the complications were resolved within 30 days of treatment (32). Thus, of previously published studies, many have demonstrated that IRE is a safe and efficient therapeutic method for the treatment of patients with pancreatic and colorectal liver metastases (36).

The limitations of the present study are as follows: No biochemical, hematologic or immunologic factors other than ALT, AST and serum bilirubin were investigated. Although the long-term results are as yet unknown, the future of IRE is promising; however, significant concerns remain regarding its safety over an extended period of time.

To conclude, IRE for primary and metastatic liver cancer may be considered safe in terms of its short-term effects on liver function; however, further study is required to confirm the findings of the present study.

Funding

The present study was supported by Scientific and Technological Plan, Guangdong province, China (grant no. 2016A020216018) and International Foundation for Sciences of Guangzhou Fuda Cancer Hospital (grant no. Y2016-ZD-008).

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

LN, MA and AMQ were responsible for study conception and design. MA and AMQ contributed to the acquisition and interpretation of data; JC drafted the work and revised the manuscript. JC and KX acquired and interpreted the data. All the authors approved the article for publication and KX agreed to be accountable for all the aspects of the work including its integrity.

Ethics approval and consent to participate

The present study was approved by the Regional Ethics Committee of Guangzhou Fuda Cancer Hospital, China. Written informed consent was obtained from all the patients, and the study protocols were in accordance with the tenets of the Declaration of Helsinki.

Patient consent for publication

Participants gave informed consent for data sharing.

Competing interests

The authors declare that they have no competing interests.