Figure 1.

Characterization of the formation and activation of NTri-SAAP. (A) Native PAGE analysis of the stepwise addressability of NTri-SAAP. (Lane 1, SAAP; Lane 2, NTri; Lane 3, SAAP and SA; Lane 4, SAAP, SA and NTri). (B) Flow cytometry assays of CEM or Ramos cells after incubation with different probes in PBS with Mg²⁺ supplied. (C) The corresponding histogram of the fluorescence ratios of CEM to Ramos cells for probes in (B). Error bars represent standard deviations from three repeated experiments.