Table 4.

Comparison of key amino acid residues in the substrate-binding site and predicted enzymatic specificities of granzymes from different species

ᅟ

Based on data from (Odake et al. 1991; Kam et al. 2000; Sattar et al. 2003), with additional data on bovine and pig in red from the present study. P1 (the residue of the substrate) specificity refers to primary substrate specificity, which is determined by primary substrate-binding pocket called S1 binding sites of the protease. Extended substrate specificity is determined by secondary substrate-binding pockets, called S2 and S3 binding sites of the protease. The predicted enzymatic functions and P1 specificity of cattle and pig granzymes are highlighted by asterisk. The sequence residue numbering is based on the chymotrypsinogen numbering

Hs Homo sapiens, Mm Mus musculus, Bt Bos Taurus, Ss Sus scrofa, GZM granzyme