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. 2018 Sep;24(9):1214–1228. doi: 10.1261/rna.066373.118

FIGURE 2.

FIGURE 2.

Residues 1-265 of Utp14 interact with proteins associated with helix 26. (A) Proteins that bind near Utp14 crosslinking sites in the SSU processome (PDB: 5WYJ). Utp22 (green), Rps1 (blue), and Rps7 (magenta) are shown. Pno1 (cyan) is also shown for perspective. Utp14 crosslinking sites are shown in red (18S rRNA) and orange (5′-ETS). 18S rRNA (gray), 5′-ETS (yellow), and U3 (light blue) are shown in surface representations. (B) Yeast two-hybrid interaction data between Utp14 and Utp22 and Utp14 and Rps1 are shown. Strains carrying the indicated constructs were patched onto Leu− Trp− (L−W−) and Leu− Trp− His− (L−W−H−) media supplemented with 3-Amino-1,2,4-triazole (3AT) as indicated (BD, GAL4BD; AD, GAL4AD). A cartoon of the Utp14 constructs indicating amino acid positions is shown to the right.