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. 2018 Sep;24(9):1214–1228. doi: 10.1261/rna.066373.118

FIGURE 6.

FIGURE 6.

The rRNA processing intermediates associated with mutant Utp14 particles. (A) A cartoon of rRNA processing and oligos used to detect intermediates. The sequences for the probes are: 5′-A0 (5′-GGTCTCTCTGCTGCCGGAAATG-3′), A0-A1 (5′-CCCACCTATTCCCTCTTGC-3′), D-A2 (5′-TCTTGCCCAGTAAAAGCTCTCATGC-3′), A2-A3 (5′-TGTTACCTCTGGGCCCCGATTG-3′). (B,C) Northern blots for rRNA processing intermediates affinity-purified via (B) TAP-tagged Utp14 full length and truncation mutants or untagged wild-type (mock) and (C) TAP-tagged Enp1 from cells depleted of Utp14 or Dhr1, conditionally expressing Utp14 truncation mutants or from untagged Enp1 (mock). Images were captured on a Typhoon FLA9500 and processed in ImageJ.