Figure 6.
Effect of LA on pro-inflammatory cytokine levels in HMC-1 cells. HMC-1 cells (3 × 105) were pretreated with LA at concentration of 400 μg/mL for 1 h, and then stimulated by PMA plus A23187 for 7 h. The levels of IL-6 and TNF-α in the supernatant were assessed using ELISA method. B, PBS-treated cells; C, PBS-treated, and then PMA plus A23187-stimulated cells. Each datum represents the mean ± S.E.M. of three independent experiments. * p < 0.05; significantly different from the control (PBS-treated, and then PMA plus A23187-stimulated cells) value.