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. 2018 May 11;23(5):1148. doi: 10.3390/molecules23051148

Figure 3.

Figure 3

Effect of Pte and 4MR on lipopolysaccharide (LPS)-induced (A) nitrite production and (B) iNOS mRNA expression in RAW264.7 cells. Cells were cotreated with both LPS (0.1 µg/mL) and Pte (5 µM) or 4MR (5 µM) for 24 h. The NO levels in the culture medium were determined using the nitrate reductase method. The mRNA expression levels were evaluated by qRT-PCR and normalized to the 18S levels. The data were representative of the three independent experiments and presented as the mean ± SEM. ### p < 0.001 compared to control; *p < 0.05, ** p < 0.01, and *** p < 0.001 compared to LPS alone.