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. 2018 Jul 27;9(58):31231–31243. doi: 10.18632/oncotarget.25782

Figure 4. m6A levels impact cellular migration in transformed, but not immortalized HMECs.

Figure 4

Wound healing scratch assay of transformed (A) and immortalized (B) HMECs. HMECs were transfected with siRNA targeting METTL3 and METTL14 (M3/14 KD), plasmids overexpressing METTL3 and METTL14 (METTL3/14 OE), plasmid expressing ALKBH5 shRNA (ALK KD), or control siRNAs/plasmids and allowed to grow to confluency. After 8 hours of serum starvation, a scratch was made with a p200 tip, cells were washed and initial pictures (0 Hr) taken. Cells were then incubated in serum free media in either normoxic or hypoxic conditions for 24 hours before a second set of pictures was taken (24 Hr). Migration was quantified by measuring the area of cell coverage of three representative fields at 0 and 24 hours. *P ≤ 0.05 by Unpaired Student's t-test. Error bars represent SEM of 3–4 experiments.