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. 2018 Jul 20;14(8):1376–1397. doi: 10.1080/15548627.2018.1476809

Figure 7.

Figure 7.

The HCK-CXCL8-IL23A signal axis of ESCs regulates FCGR3− NK cells differentiation. (a) KEGG database-derived bioinformatics analysis about the relationship between HCK downstream cytokines (CXCL8, IL23A and IL1A), Fc receptors FCGR3A and FCGR3B. (b) (left) After coculture with Ctrl-ESC, HCK (OE)-ESC, CXCL8 protein (100ng/ml) and/or IL23A (OE)-ESC for 48 h; (right) After coculture with Ctrl-ESC, siHCK-ESC, anti-CXCL8 (2 ug/ml) and/or siIL23A-ESC for 48 h, the ratio of FCGR3−:FCGR3+ NK cells (n = 6, right) were analyzed by FCM (one-way ANOVA). (c,d) The weights of EMS-like lesions from C57BL/6 EMS mice (n = 8 mice/group) were counted after treatment with or without intraperitoneal injection of anti-mouse IL23A neutralizing antibody (anti-IL23A, 0.1 mg/kg/d) on day 3 and day 10 (Student t test). (e,f) The concentration of CXCL8 and IL23A in PF from healthy control (n = 20) and EMS patients (stage III and IV, n = 20), supernatants of ESCs from healthy control (n = 10) and EMS patients (stage III and IV, n = 10) were detected by ELISA (Student t test). CXCL8, recombinant human CXCL8 protein; anti-CXCL8, anti-human CXCL8 neutralizing antibody. Data are expressed as the mean± SEM. *P < 0.05, **P < 0.01and ***P < 0.001 (compared to Ctrl-ESC); #P < 0.05 and ###P < 0.001 (compared to HCK (OE)-ESC or siHCK-ESC group).