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. 2018 Aug 21;7:e36494. doi: 10.7554/eLife.36494

Figure 9. CNO-mediated bilateral activation of hM3Dq-containing LHA CART neurons leads to increased body skin temperature in Cartptcre/cre and Cartptcre/+ mice.

Temperatures of the interscapular brown adipose tissue (BAT) (A, B), the lumbar back (D,E), the tail (G,H) and differences between BAT and lumbar temperatures (J, K) measured by high-sensitivity infrared imaging 1, 3 and 6 hr after i.p. injection of saline or CNO. The difference of these parameters between Cartptcre/cre and Cartptcre/+ mice was assessed by subtracting the value of CNO from that of saline at the corresponding time point for each mouse (C,F, I, L). Data are means ± SEM. n = 6–7; *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001 for saline versus CNO treatments.

Figure 9.

Figure 9—figure supplement 1. CNO does not exert any effects on body skin temperature in the absence of hM3Dq-expression in Cartptcre/cre, Cartptcre/+ and wild type mice.

Figure 9—figure supplement 1.

Temperatures of the interscapular brown adipose tissue (BAT) (A, B, C), the lumbar back (D, E, F), the tail (G, H, I) measured by high-sensitivity infrared imaging 1, 3 and 6 hr after i.p. injection of saline or CNO of Cartptcre/cre (A, D, G), Cartptcre/+ (B, E, H) without hM3Dq-injection and wild type mice receiving bilateral LHA→hM3Dq but lacking Cre-recombinase expression (C, F, I). Data are means ± SEM. n = 8.