Ectoderm explants were dissected at stage 9-10 from Tg(sox10:GFP) uninjected embryos, and from those injected with indicated RNAs. The explants were cultured until the time when control embryos reached stage 28 and fixed for imaging (A-H). Chordin RNA was used either alone (150 pg, B) or in combination with different wnt RNAs. N-Daam1 (850 pg) inhibits the response to wnt11a (2 ng, E, F), wnt11b (2 ng, G, H), but not to wnt8 (700 pg, C, D) or wnt3a (525 pg, I and data not shown). I, Frequencies of GFP-positive explants (%) are representative of two different experiments. Total number of animal caps is shown above each bar.