FIG 8 .

WRE occurrences in the upstream regions of wetA orthologs in representative fungi. WRE occurrences were identified in a series of regions located upstream of wetA orthologs. (A) Numbers to the left of the sequence indicate at what position relative to the translation start site the sequence shown begins. The sequences shown are from 15 bp upstream of the WRE occurrence that was identified by FIMO (40) with the lowest P value to 14 bp downstream of the WRE occurrence. Bases are colored black if they are conserved in at least 60% of the species. Green—Aspergillaceae. Orange—Trichocomaceae. Blue—Onygenales. Purple—Sodariomycete. (RC)—reverse complement. (B) ChIP-qPCR analysis demonstrated that WetA occupies wetA promoters in A. nidulans, A. fumigatus, and A. flavus. Data are presented as fold change compared with rabbit IgG-enriched DNA fragments. Data presented are means ± SD, n = 3.