Fig. 5. Phosphorylation status of the Tyr176 residue of PTPIP51, activation status of MAPK signaling and activation status of Akt signaling under Gefitinib, Lapatinib, Neratinib, and Mubritinib treatment.
Immunobloting of phospho Tyr176 PTPIP51. Cell were treated with the indicated concentrations of the four different TKIs. The multiple bands are due to the different isoforms of PTPIP51. For evaluation only the 52 kDa isoform of PTPIP51 was used. The immunoblots were normalized to the stain-free blot