Figure 1.
The CBS7/9 boundary demarcates active and repressive chromatin domain and maintains ectopic expression of posterior HOXA genes. ChIP analysis of H3K4me3 (A), H3K79me2 (B), H3K9ac (C), and H3K27me3 (D) across the HOXA locus in 3 subtypes of primary AML. Patient 974 possesses NPM1C+ and FLT3-ITD mutations, patient LPP4 contains MLL rearrangement, and patient 1306 has gain of MLL copy number. (E) ChIP-seq analysis of CTCF binding at the HOXA locus obtained from the NCBI GEO public database (accession number GSM1335528). (F) Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analysis of HOXA gene expression in 3 subtypes of primary AML (974, LPP4, and 1306).