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. 2018 Aug 6;7:e36851. doi: 10.7554/eLife.36851

Figure 2. The microtubule triplet undergoes significant changes along its proximal-distal axis.

The microtubule triplet sub volumes were grouped based on position along the proximal-distal axis of the centriole, and then averaged together to generate maps that represented 100 nm blocks of the centriole. (a) and (b) There were no major structural differences between the first two groups (0–100 nm and 100–200 nm); complete triplet microtubules were bound by an A-C linker and the pinhead. (c) In the third group (200–300 nm) the A-C linker, part of the C-tubule and the pinhead were no longer obvious in the map, but a new density (gold double arrowhead) occupied the space previously occupied by the pinhead. (d) In the distal centriole (300–400 nm) a partial, though less complete C-tubule persisted as did the novel density (gold double arrowhead). Scale bars are 25 nm.

Figure 2.

Figure 2—figure supplement 1. Unbiased classification of the subvolume population suggested centriole domains.

Figure 2—figure supplement 1.

(a) Our analysis of the CHO average suggested that several features, including the pinhead, were at less than 100% occupancy in the average. All subvolumes contributing to the whole-population average were classified based on the presence or absence of the pinhead, using a mask specific to the pinhead (dashed circle). (b) This classification yielded several classes, but the most populous class yielded an average that was enriched for pinhead-containing subvolumes. (c) A representative centriole refit showed that pinhead-containing subvolumes clustered together, suggesting that the centriole had distinct domains along its proximal-distal axis. Scale bar is 25 nm in (a) and (b), and 100 nm in (c).
Figure 2—figure supplement 2. Fine-grain averages along the entire length of the CHO centriole.

Figure 2—figure supplement 2.

Subvolumes were averaged in 50 nm bins along the length from 0 to 400 nm. Any additional subvolumes from centrioles that were longer than 400 nm in length were grouped together in one class, and represent the distal-most part of the CHO centriole. Scale bar is 25 nm.