Figure 1. Anatomical connection between DCN and Area X.
(A) Injection sites of Dextran Alexa 488 (green, top panel) and Dextran Alexa 594 (magenta, bottom panel, sagittal sections). Dotted line delimits Area X (top panel). Scale bar: 100 µm. (B) Labelling in the dorsal thalamic zone (DTZ) showing efferent cerebellar fibers (magenta) and cell bodies of neurons in DTZ (green). Large labelling of efferent fibers from Area X nucleus was also found in DLM, as the tracer is anterogradely and retrogradely transported. The dotted line in B delimits nucleus DLM, while the white square indicates magnification location for C. The large dotted line delimits the brain slice contour. Scale bar: 100 µm. DLM: dorsolateral nucleus of the anterior thalamus. (C) Magnification of the dorsal thalamic zone. Cerebellar fibers are labelled in magenta, and somata are labelled in green and indicated with arrowheads. Scale bar: 100 µm. (D) Injection sites of cholera toxin B in Area X (green, left panel) and Dextran Alexa 594 in DCN (magenta, right panel). Dotted lines delimit Area X (left panel) and all three DCN (right panel). The large dotted line delimits the brain slice contour. Retrograde labeling of Purkinje cells projecting to the DCN targeted by dye injection can be observed (right panel, arrowheads). Scale bar: 100 µm. (E–F): Close contacts were observed in the dorsal thalamic zone (DTZ, scale bars: 100 µm). The dotted line in E delimits nucleus DLM, while the white square in E and F indicates magnification location. Efferent fibers from Area X in DLM appear as diffuse green labeling in this nucleus, while green cell somas in DTZ reflect afferent neurons. Magenta-labeled fibers from the DCN surround Area X-projecting neurons in DTZ. (G): Three examples of close contacts between fibers from the DCN (magenta, middle panel) and soma of neurons projecting to Area X (green, left panel) in DTZ. Each panel in G corresponds to a magnification of squares indicated in F. The merge suggests an anatomical connection (right panel). Scale bar: 2 µm. (G) Injection sites of Dextran Alexa 594 in DTZ. The large dotted line delimits slice contours, and the dotted circle represents DLM. Scale bar: 100 µm. (H) Two examples of retrograde labelling in the lateral DCN following DTZ injection showed in G. Both examples are from the same animal, at two different depths. (I) Arrowheads indicate DCN cell soma labelled. The dotted line delimits the lateral DCN contours. Scale bar: 20 µm (J) Example of anterograde labelling in Area X. Only fibers (but no soma) were observed in Area X after DTZ injection. Scale bar: 2 µm.
Figure 1—figure supplement 1. Magnification of Purkinje cell labelling.
