FIG 3 .

Deletion of cysD abrogates calcium-induced P_brpA expression. (A) The wild-type (WT), cysD mutant (ΔcysD), and complemented strains (ΔcysD-C) were grown on LB^Ca containing X-Gal. (B) The same strains were grown in LB ± 10 mM CaCl₂ (+Ca) for quantification of P_brpA lacZ expression. Statistically significant differences among the samples (P < 0.001 as determined by one-way ANOVA followed by pairwise comparisons with a Bonferroni adjustment) are indicated by different symbols above each bar.