Table 1. Primers, their sequences and annealing temperatures used for RT-qPCR.
| Primer | Primer sequence (5′-3′) | Annealing temperature (°C) | Manufacturer | |
|---|---|---|---|---|
| E-cadherin | forward | TGCTCTTGCTGTTTCTTCGG | 55 | RealTimePrimers |
| reverse | TGCCCCATTCGTTCAAGTAG | |||
| GAPDH | forward | TCCATGACAACTTTGGTATCGTGG | 58 | Eurofins |
| reverse | GACGCCTGCTTCACCACCTTCT | |||
| L1CAM | forward | GAACTGGATGTGGTGGAGAG | 58 | RealTimePrimers |
| reverse | GAGGGTGGTAGAGGTCTGGT | |||
| Nanog | forward | ACCTACCTACCCCAGCCTTT | 58 | RealTimePrimers |
| reverse | CATGCAGGACTGCAGAGATT | |||
| Nestin | forward | GAAACAGCCATAGAGGGCAAA | 58 | Eurofins |
| reverse | TGGTTTTCCAGAGTCTTCAGTGA | |||
| TNF-α | forward | TCCTTCAGACACCCTCAACC | 58 | Eurofins |
| reverse | AGGCCCCAGTTTGAATTCTT | |||
| Vimentin | forward | TCCAAGTTTGCTGACCTCTC | 58 | RealTimePrimers |
| reverse | TCAACGGCAAAGTTCTCTTC | |||
| Zeb1 | forward | TCCATGCTTAAGAGCGCTAGCT | 61 | Eurofins |
| reverse | ACCGTAGTTGAGTAGGTGTATGCCA |