Skip to main content
. 2018 Aug 3;9(60):31620–31636. doi: 10.18632/oncotarget.25824

Figure 5. Loss of expression of the Hippo kinases at the RNA level and reconstitution with 5-azacytidine in sarcoma cell lines evaluated by quantitative RT-PCR.

Figure 5

Loss of expression was defined as a two-fold or greater decrease of expression of the Hippo kinases (0.5 fold decrease in expression as compared to GCT). Increase in expression was defined as a two-fold or greater increase in expression after treatment with 10 μM 5-azacytidine. (A) MST1 expression is decreased at the RNA level in 5 of 12 sarcoma cell lines (42%). MST2 expression is decreased in 3 of 12 sarcoma cell lines (25%). LATS1 expression is decreased in 1 of 12 sarcoma cell lines (8%). No decrease in LATS2 expression is detected (0%). (B) TCGA data demonstrating that MST2 expression is inversely proportional to CpG island methylation (Spearman's correlation coefficient r = −0.4). (C) MST1 expression is increased after treatment with 5-azacytidine in 1 of 12 cell lines (8%). MST2 expression is increased after treatment in 2 of 12 cell lines (17%). Neither LATS1 nor LATS2 expression is increased in any (0%) of the cell lines after treatment with 5-azacytidine. Statistical analysis is shown for cell lines demonstrating a two-fold or greater decrease in expression. Statistical significance determined by two-tailed t-test. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001.