Abstract
Objective
Prior reports suggested that infection with Helicobacter pylori was associated with respiratory diseases; pathogenetic mechanisms however, were not defined. We tested the hypothesis that VacA, an exotoxin of Helicobacter pylori, a gastric pathogen, was aspirated into the lung and could stimulate secretion of inflammatory cytokines by lung epithelial cells.
Methods
The presence of VacA was determined by immunohistochemistry in surgical lung biopsy tissue samples from 72 patients with interstitial pneumonia. The effects of VacA on A549 human alveolar epithelial adenocarcinoma cells and normal human bronchial epithelial cells were determined. After incubation with VacA, the secretions of cytokines were measured by Multiplex Luminex® Assays.
Results
VacA was detected with anti-VacA antibodies in bronchial epithelial cells and alveolar epithelial cells from 10 of 72 patients with interstitial pneumonia. VacA was more prevalent in lungs of patients with collagen vascular disease-associated interstitial pneumonia than in those of patients with idiopathic pulmonary fibrosis, nonspecific interstitial pneumonia and cryptogenic organizing pneumonia. Incubation of A549 cells and normal human bronchial epithelial cells with VacA for 24 h was cytotoxic, and resulted in vacuolation. VacA induced interleukin-8 production by A549 cells and normal human bronchial epithelial cells and interleukin-6 production by A549 cells. Based on multiplex screening, interleukin-8 and interleukin-6 were the primary secretory products induced by VacA.
Conclusions
Helicobacter pylori VacA is present in human lung and can induce interleukin-8 and interleukin-6 production by human lung cells. VacA could have a role in the pathogenesis of respiratory diseases by its cytotoxic effects and by inducing the secretion of interleukin-8 and interleukin-6 by targeted airway epithelial cells.
Keywords: Helicobacter pylori, VacA, A549 cells, normal human bronchial epithelial cells, interleukin-8, interleukin-6
Introduction
Helicobacter pylori (H. pylori) has been shown to be causally related to some digestive diseases, such as gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. Recently, however, H. pylori infection has been reported to be associated with extra-digestive diseases [1]. A high seroprevalence of H. pylori was observed in patients with bronchiectasis [2] and chronic bronchitis [3]. Further, patients with idiopathic pulmonary fibrosis (IPF) having anti-H. pylori antibodies in serum showed significantly lower pulmonary function and increased mortality compared to patients with IPF but without anti-H. pylori antibodies in serum [4]. These reports suggest that H. pylori could be associated with respiratory diseases, however pathogenetic mechanisms for the effects of H. pylori were not defined.
It is possible that H. pylori could be aspirated into the respiratory tract from oropharynx or the gastric reservoir and cause respiratory disease. This hypothesis, however, appears unlikely since H. pylori deoxyribonucleic acid (DNA) was not detected by polymerase chain reaction (PCR) in bronchoalveolar lavage fluids and lung tissue samples [5], and an anti-H. pylori antibody was not reactive with bronchial mucosa [6]. Furthermore, it appears unlikely that H. pylori could proliferate in the environmental conditions found in human lung. Therefore, we hypothesized that H. pylori infection raises the risk of respiratory diseases indirectly, rather than directly, by means of systemic inflammatory and/or autoimmune responses, and/or by aspiration into the lung of H. pylori products, such as exotoxins. Vacuolating cytotoxin (VacA) is the major exotoxin of H. pylori [7,8], and induces vacuolation in the cytoplasm of susceptible mammalian cells. Further, VacA has pleiotropic effects on target cells, including membrane depolarization, mitochondrial dysfunction, autophagic process, activation of mitogen-activated protein kinases, inhibition of T cell function and cell death [9].
We hypothesized that H. pylori VacA could be aspirated into the lung and stimulate secretion by lung epithelial cells of inflammatory cytokines. Here, we investigated the presence of VacA in human lung and whether inflammatory cytokine production could be induced by VacA in A549 human alveolar epithelial adenocarcinoma cells and normal human bronchial epithelial (NHBE) cells.
Methods
Study population
The protocol of this retrospective study was approved by the Institutional Review Board of Nagasaki University Hospital and the Ethics Committee, Nagasaki University Graduate School of Biomedical Sciences, IRB00009218. We evaluated 72 patients with interstitial pneumonia who underwent surgical lung biopsy in Nagasaki University Hospital between 1983 and 2011. Twenty-two patients were diagnosed with IPF, 19 with nonspecific interstitial pneumonia (NSIP), 8 with cryptogenic organizing pneumonia (COP) and 23 with collagen vascular disease-associated interstitial pneumonia (CVD-IP). The idiopathic interstitial pneumonias were diagnosed according to the consensus criteria of the American Thoracic Society/European Respiratory Society [10]. The diagnoses associated with CVD-IP were rheumatoid arthritis (RA; n = 6), systemic sclerosis (SSc; n = 5), Sjogren’s syndrome (SjS; n = 5), polymyositis/dermatomyositis (PM/DM; n = 3), mixed connective tissue disease (MCTD; n = 2), RA/SjS overlap (n = 1), and SjS/DM overlap (n = 1).
Immunohistochemistry
Immunohistochemistry was performed in 4-μm paraffin sections. The primary antibodies used for the immunohistochemical studies included an anti-Helicobacter pylori antibody (Dako, Glostrup, Denmark) and rabbit anti-VacA antibodies, which were prepared as described previously [11]. Control studies were performed using rabbit immunoglobulin (Dako) as the primary antibodies. Immunohistochemistry was performed using the EnVision™ System (Dako) according to the manufacturer’s instructions.
Incubation of cultured cells with VacA
A549 cells (American Type Culture Collection, Rockville, MD, USA) and NHBE cells (CC-2541, Lonza, Walkersville, VA, USA) were maintained in, respectively, Dulbecco’s modified minimal essential medium (DMEM; Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Invitrogen) and bronchial epithelial growth medium (BEGM; Lonza) in a humidified incubator (5% CO2 at 37°C).
The toxin-producing H. pylori strain, ATCC 49503, was the source of VacA, which was purified as described previously [12].
To quantify cytokine production, A549 cells were seeded in 6-well plates at a density of 3.0 × 105 per well and NHBE cells in 12-well plates at a density of 1.0 × 105 per well. At subconfluence, serum-free medium was added to the wells. Cells were subsequently stimulated for 3, 6, 12, and 24 h with 1, 10, 30, 60, or 120 nM VacA or heat-inactivated VacA (100°C, 10 min; iVacA control). These VacA concentrations have been used for the purpose of investigating the effects of toxin on gastric epithelial cells [13]. Changes in cell morphology were assessed by phase-contrast light microscopy.
Cytokine levels in supernatants from cells incubated with VacA
Cytokines in supernatants were measured by Multiplex Luminex® Assays. The Cytokine Human 10-Plex Panel (granulocyte macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-γ, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 and tumor necrosis factor (TNF)-α) was purchased from Life Technologies (LHC0001). 96-well plates, reagents and wash solutions provided or recommended by the manufacturer were used and all data were analysed as recommended by the manufacturers. All measurements were performed in duplicate, and the values reported are the means of 4 experiments.
Statistical analysis
The values of continuous variables were expressed as median (range). Differences between 2 groups were determined by Mann-Whitney’s U-test. Differences among groups were determined by one-factor analysis of variance for continuous variables, and Pearson’s chi-square test for categorical variables, as appropriate. If a significant difference was found by one-factor analysis of variance, pair-wise comparison was performed using the Scheffë method. Statistical analysis was performed using Stat ViewJ-5.0 (SAS Institute, Cary, NC, USA). A p-value < 0.05 was considered statistically significant.
Results
Histology and immunohistochemistry
To verify the effectiveness of the antibodies, we performed immunohistochemistry with anti-VacA and anti-H. pylori antibodies using gastric paraffin sections from patients with H. pylori infection (representative results are shown in Fig. 1a–c). Anti-H. pylori antibodies recognized H. pylori in gastric sections (Fig. 1b). Similarly, VacA was identified in H. pylori as well as in the vicinity of the bacteria (Fig. 1c). Control studies using rabbit immunoglobulin did not reveal any immunoreactive cells (Data not shown).
To identify and localize VacA in human lung tissues, we performed immunohistochemical analysis for VacA in lung paraffin sections from patients with different types of interstitial pneumonias. VacA was detected in the lung tissues in 10 of 72 patients (13.9%) by immunohistochemistry (representative results are shown in Fig. 1 d–i). VacA was identified in alveolar epithelial cells (Fig. 1e) and in bronchial epithelial cells (Fig. 1h). Control studies using rabbit immunoglobulin did not reveal any immunoreactive cells (Fig. 1f, 1i). In this study, gastric (Fig. 1a–c) and lung tissues (Fig. 1 d–f) were obtained from the same patient.
Comparison of patient characteristics between the VacA-positive and -negative groups
Patient demographic data and diagnosis in the VacA-positive (n = 10) and VacA-negative (n = 62) groups are shown in Table 1. The diagnoses of VacA-positive cases were IPF (n = 1), NSIP (n = 2) and CVD-IP (n = 7). The diagnoses of collagen vascular diseases in the VacA-positive group included SjS (n = 2), and PM/DM, SSc, RA, MCTD, and SjS/DM overlap (n = 1, respectively). There was a significant difference in the diagnoses between the 2 groups (p=0.04), VacA was more prevalent in the lungs of patients with CVD-IP than those of patients with IPF, NSIP and COP. Detailed chart review revealed that there were no significant differences in other clinical characteristics, including survival, results of pulmonary function tests, arterial blood gas analysis, serological findings and bronchoalveolar lavage fluid findings (Data not shown).
Table 1.
VacA–positive | VacA–negative | P-value | |
---|---|---|---|
Subjects | 10 | 62 | |
Age (years) | 54.5 (40−69) | 60 (28−74) | NS |
Sex (male:female) | 4:6 | 33:29 | NS |
Diagnosis | < 0.05 | ||
IPF | 1 | 21 | |
NSIP | 2 | 17 | |
COP | 0 | 8 | |
CVD-IP | 7 | 16 |
IPF: idiopathic pulmonary fibrosis, NSIP: nonspecific interstitial pneumonia, COP: cryptogenic organizing pneumonia, CVD-IP: collagen vascular disease-associated interstitial pneumonia
VacA-induced vacuolation
Incubation of A549 and NHBE cells with 120nM VacA resulted in vacuolation (Fig. 2a, 2c), which was not observed in cells incubated with 120 nM iVacA (Fig. 2b, 2d), suggesting that vacuolation in cells was a specific response to VacA protein.
VacA-induced cytokine production
A549 and NHBE cells were incubated with 120 nM VacA or iVacA for 24 h. As shown in Fig. 3a, after a 24-h incubation with 120 nM VacA or iVacA, 1.9- and 3.6-fold increase were observed in IL-8 and IL-6 concentrations in the medium of A549 cells, respectively. IL-1β and GM-CSF production were also induced by VacA although these were only a slight induction. After a 24-h incubation with 120 nM VacA or iVacA, 17.2-fold increase was observed in IL-8 concentration in the medium of NHBE cells (Fig. 3b).
A549 cells were incubated with the indicated concentrations of VacA or iVacA (0–120 nM) for 24 h, VacA increased IL-8 (Fig. 4a) and IL-6 (Fig. 4b) in a concentration-dependent manner. Additionally, A549 cells were incubated with 120 nM VacA or iVacA for the indicated times (0–24 h). Stimulation with 120 nM VacA resulted in a time-dependent increase in IL-8 (Fig. 4d) and IL-6 (Fig. 4e) production. Regarding IL-1β and GM-CSF, there were no concentration- and time-dependent changes (Data not shown).
NHBE cells were also incubated with the indicated concentrations of VacA or iVacA (0–120 nM) for 24 h and with 120 nM VacA or iVacA for the indicated times (0–24 h). VacA increased IL-8 in a concentration-(Fig. 4c) and time-dependent manner (Fig. 4f).
Discussion
This is the first study to demonstrate that H. pylori VacA is present in human lung tissues and that VacA induces IL-8 production by A549 and NHBE cells and IL-6 production by A549 cells. The present study also showed that VacA was more prevalent in lungs of patients with CVD-IP than those of patients with IPF, NSIP and COP.
Association between respiratory diseases and gastro-esophageal reflux (GER) has long been recognized [14]. GER has been documented to be highly prevalent in patients with a variety of lung diseases including patients with IPF, cystic fibrosis and CVDs [15]. Chronic microaspiration (i.e., tracheobronchial aspiration of small amounts of gastric secretions) secondary to GER has been hypothesized to cause repetitive subclinical injury to the lung, leading to pulmonary fibrosis [16]. Previous reports showed the presence of high levels of pepsin [17–21] and bile acids [22,23] in the bronchoalveolar lavage fluid of patients with lung transplants and children with GER or cystic fibrosis, suggesting that gastric contents could be aspirated into the lung by microaspiration without any distinct clinical episode. It has been suggested that pepsin or bile acids aspirated through GER may be a potential contributor to lung allograft dysfunction [20–22]. Moreover, suppression of GER in patients with IPF was associated with a smaller decrease in forced vital capacity [24] and a lower radiological fibrosis score on high-resolution computed tomography, and was an independent predictor of longer survival [25]. Other substances in gastric juice as yet unidentified may also contribute to the pathogenesis of respiratory dysfunction and disease. Numerous studies have also shown that H. pylori can be detected in dental plaque and saliva of human subjects, suggesting that the oral cavity may be an extra-gastric reservoir of H. pylori [26]. These findings suggest that VacA could be aspirated into the lung from stomach and/or oral cavity. In fact, in the present study, VacA was detected in the bronchial and alveolar epithelial cells, which are easily accessible following aspiration. Further studies are needed to elucidate the VacA entering mechanisms.
VacA is known to cause vacuoles in human gastric cancer cell lines AZ-521 and AGS, but not in human promyeloblastic cell line HL-60 [11], VacA-induced vacuolation thus appears to be cell-type specific. We showed that VacA causes vacuoles in A549 and NHBE cells. In addition, vacuolation were not observed with heat-inactivated VacA, indicating that these vacuolation were specific to the native VacA protein. However, there was no evidence of vacuolation in VacA immunoreactive cells in human lung tissues and clinical significance of this phenomenon still remains unknown.
Because of its prevalence and ability to affect human immune function, many researchers have hypothesized that H. pylori might contribute to the systemic rheumatic diseases development. Previous reports have suggested a positive relationship between H. pylori infection and various autoimmune diseases [27]. Some evidences suggest that eradication of H. pylori may lead to an improvement of autoimmune disorders, such as immune thrombocytopenia [28], SSc [29] and autoimmune thyroid disease [30]. The present study showed that VacA was more prevalent in lungs of patients with CVD-IP than that of patients with IPF, NSIP and COP. Although the exact role of H. pylori in the pathogenesis of these diseases is still unclear, these findings imply the possible association of H. pylori and autoimmune disorders.
In the present study, we investigated whether several kinds of cytokines (GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 and TNF-α) could be induced in A549 and NHBE cells by VacA. Interestingly, VacA induced IL-8 production by A549 and NHBE cells and IL-6 production by A549 cells. Other cytokines were not induced by VacA. These findings suggest that induction of IL-8 and IL-6 in airway epithelial cells are specific reaction against VacA stimulation. IL-8 is expressed in a variety of inflammatory respiratory diseases, including IPF [31], CVD-IP [32] and bronchiectasis [33]. Interestingly, increased plasma IL-8 levels were associated with significantly worse outcomes in IPF [31]. IL-6 is also known to induce interstitial pneumonia [34–36]. The prolonged interaction between the VacA and lung epithelial cells could trigger the release of inflammatory mediators and consequently lead to respiratory disease in predisposed individuals.
Some limitations to this study should be noted. The most important limitation of this study is that only 72 patients were enrolled. We were also not able to evaluate the serum H. pylori antibodies and the concentrations of IL-8 and IL-6 in bronchoalveolar lavage fluid in the enrolled patients because the volume of preserved samples was not sufficient. Further clinical studies involving larger cohorts of patients are warranted to elucidate whether VacA was associated with the pathogenesis of pulmonary diseases and CVDs.
In conclusion, our identification of VacA in human lung tissues of patients with interstitial pneumonia, and the demonstration that VacA induced vacuolation and production of IL-8 and IL-6 by airway epithelial cells, together with previous reports of a high H. pylori seroprevalence in several types of respiratory diseases, suggest that VacA could play a role in the pathogenesis of respiratory diseases and/or CVDs. Further studies of the immunological response to H. pylori VacA and its role in the pathogenesis of interstitial pneumonias and CVDs are warranted.
H. pylori VacA was detected in bronchial and alveolar epithelial cells of human lung.
Incubation of A549 and NHBE cells with VacA resulted in vacuolation.
Interleukin-8 and interleukin-6 production were induced by VacA in A549 cells.
Interleukin-8 production was induced by VacA in NHBE cells.
Acknowledgments
The authors thank Dr. Y. Morinaga and Prof. K. Yanagihara (Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences) for performing the Multiplex Luminex® Assays and Mr. A. Yokoyama (Nagasaki University Hospital) for technical assistance.
B.G., A.S. and J.M. were supported by the Intramural Research Program, National Institutes of Health, NHGRI, CC and NHLBI, respectively.
Abbreviations
- H. pylori
Helicobacter pylori
- NHBE
normal human bronchial epithelial
- IPF
idiopathic pulmonary fibrosis
- DNA
deoxyribonucleic acid
- PCR
polymerase chain reaction
- NSIP
nonspecific interstitial pneumonia
- COP
cryptogenic organizing pneumonia
- CVD-IP
collagen vascular disease-associated interstitial pneumonia
- RA
rheumatoid arthritis
- SSc
systemic sclerosis
- SjS
Sjögren’s syndrome
- PM
polymyositis
- DM
dermatomyositis
- MCTD
mixed connective tissue disease
- GM-CSF
granulocyte macrophage colony-stimulating factor
- IFN
interferon
- IL
interleukin
- TNF
tumor necrosis factor
- GER
gastro-esophageal reflux
Footnotes
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