FIG 4 .

Comparison of levels of transmission competence of WT, Tr-F, Tr-C, and KO parasites in A. stephensi mosquitoes. Oocyst numbers of mosquitoes fed on 8 infected mice (4 experiments with 2 mice per experiment) were pooled for each of the groups of WT, Tr-F, and Tr-C parasites. KO parasites were evaluated in only a single mosquito feeding experiment (2 mice). Horizontal red lines represent medians of oocyst numbers. The data corresponding to the rates of infection represent percent mosquitoes infected (infected mosquitoes/total number of mosquitoes dissected) and are shown at the bottom. Median oocyst numbers of WT parasites were significantly higher than those of Tr-F and Tr-C parasites. No statistically significant difference (NS) was detected between Tr-F and Tr-C parasites (P values are indicated and were determined by the Kruskal-Wallis test followed by Dunn’s test for specific group pairs). The Fisher’s exact test was used to compare the rates of infection and indicated that the differences between the results determined for WT, Tr-F, and Tr-C parasites were not significant. Note that 7 points of WT data are outside the y-axis limit.