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. 2018 Aug 15;5(8):181213. doi: 10.1098/rsos.181213

Correction to: ‘The effect of alkali-soluble lignin on purified core cellulase and hemicellulase activities during hydrolysis of extractive ammonia-pretreated lignocellulosic biomass’

Linchao Zhou, Leonardo da Costa Sousa, Bruce E Dale, Jia-Xun Feng, Venkatesh Balan
PMCID: PMC6124048  PMID: 30225091

R. Soc. open sci. 5, 171529. (Published 27 June 2018). (doi:10.1098/rsos.171529)

This correction refers to tables 2 and 3; the data of ‘EX’ and ‘βX’ (column; from nos 8 to 31) in these tables have been revised.

Table 2.

Glucan conversion after 24 h hydrolysis for two different pretreated CS samples at three different protein loadings, while keeping βG loading at 10% supplementation in all experiments. Average (avg.) and standard deviation (s.d.) were based on experiments that were done in triplicate.

no. purified enzymes
glucan conversion for EA-CS(−)
glucan conversion for EA-CS(+)
7.5 mg g−1
15 mg g−1
30 mg g−1
7.5 mg g−1
15 mg g−1
30 mg g−1
CBH I CBH II EG I EX βX avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%)
1 1 0 0 0 0 5.6 0.1 9.7 0.3 18.9 0.4 5.0 0.1 8.9 0.0 18.3 0.1
2 0 1 0 0 0 5.8 0.1 7.5 0.1 10.0 0.2 5.1 0.2 6.2 0.1 8.2 0.0
3 0 0 1 0 0 16.8 0.2 20.8 0.3 25.8 0.3 13.4 0.2 16.5 0.1 21.7 0.0
4 0 0 0 1 0 4.5 0.2 6.0 0.2 7.6 0.1 4.6 0.1 5.7 0.3 7.0 0.2
5 0 0 0 0 1 1.7 0.2 2.2 0.3 2.5 0.1 2.2 0.1 2.6 0.0 3.0 0.1
6 0.5 0.5 0 0 0 8.4 0.2 14.1 0.2 24.6 0.2 7.1 0.1 12.1 0.1 22.5 0.2
7 0.5 0 0.5 0 0 37.2 0.2 54.6 0.0 73.9 0.2 35.8 0.4 53.2 0.5 69.1 2.5
8 0.5 0 0 0.5 0 11.5 0.2 22.0 0.4 40.5 0.3 10.9 0.0 22.1 0.2 41.0 0.2
9 0.5 0 0 0 0.5 3.7 0.1 6.2 0.2 11.9 0.1 3.8 0.2 5.7 0.1 11.2 0.2
10 0 0.5 0.5 0 0 31.0 0.4 42.9 0.2 58.7 0.4 27.7 0.5 39.0 0.7 56.7 0.8
11 0 0.5 0 0.5 0 13.3 0.1 19.9 0.2 29.8 0.3 11.9 0.3 17.7 0.0 28.9 0.3
12 0 0.5 0 0 0.5 4.7 0.2 6.3 0.1 8.2 0.2 4.6 0.3 5.4 0.2 7.0 0.1
13 0 0 0.5 0.5 0 16.2 0.1 19.3 0.0 23.4 0.2 12.8 0.4 15.4 0.7 19.5 0.2
14 0 0 0.5 0 0.5 14.6 0.2 17.9 0.1 22.2 0.1 11.5 0.3 14.0 0.2 17.7 0.1
15 0 0 0 0.5 0.5 3.4 0.1 5.1 0.2 6.5 0.0 3.8 0.2 4.9 0.0 6.3 0.2
16 0.33 0.33 0.33 0 0 52.3 0.3 69.4 0.3 82.4 1.7 50.6 0.2 65.8 4.0 77.8 4.8
17 0.33 0.33 0 0.33 0 26.1 0.1 45.9 0.2 71.4 0.9 25.0 0.4 45.7 0.8 64.0 0.4
18 0.33 0.33 0 0 0.33 7.0 0.2 11.5 0.1 20.0 0.2 5.9 0.0 9.5 0.2 17.8 0.2
19 0.33 0 0.33 0.33 0 41.5 0.3 58.9 0.1 79.4 1.4 38.9 0.2 56.4 1.4 71.1 3.4
20 0.33 0 0.33 0 0.33 33.6 0.1 49.7 0.5 71.2 1.7 31.7 0.3 48.0 0.5 63.4 1.8
21 0.33 0 0 0.33 0.33 8.8 0.2 16.3 0.2 30.7 0.0 8.7 0.1 16.1 0.1 30.7 0.5
22 0 0.33 0.33 0.33 0 31.9 0.1 43.6 0.2 58.8 0.5 29.2 0.2 40.0 0.5 56.1 1.3
23 0 0.33 0.33 0 0.33 27.1 0.2 37.7 0.1 53.0 0.5 23.7 0.5 33.9 0.4 49.3 0.5
24 0 0.33 0 0.33 0.33 11.0 0.2 16.5 0.3 24.7 0.2 9.5 0.2 15.1 0.0 23.3 0.3
25 0 0 0.33 0.33 0.33 14.2 0.4 17.3 0.3 21.0 0.2 11.2 0.5 13.2 0.3 16.6 0.4
26 0.25 0.25 0.25 0.25 0 60.8 0.3 78.0 0.4 93.9 1.5 58.8 0.4 74.4 2.2 80.4 2.5
27 0.25 0.25 0.25 0 0.25 49.8 1.2 68.7 0.8 84.9 0.8 47.0 1.3 63.2 0.5 73.9 1.8
28 0.25 0.25 0 0.25 0.25 20.9 0.2 38.4 0.3 63.7 1.1 20.3 0.5 38.4 0.1 61.5 0.8
29 0.25 0 0.25 0.25 0.25 36.5 0.3 53.0 0.1 73.8 0.6 35.3 1.4 52.0 0.0 72.5 1.3
30 0 0.25 0.25 0.25 0.25 28.2 0.3 39.0 0.4 53.9 0.6 25.6 0.4 36.2 0.9 51.9 1.4
31 0.2 0.2 0.2 0.2 0.2 55.0 0.5 75.7 0.5 89.8 1.4 53.9 0.7 74.1 1.9 89.5 3.9
32 Ctec2/Htec2 7/3 73.5 0.1 88.5 0.7 99.5 1.4 65.5 1.3 81.0 0.7 94.1 1.9

Table 3.

Xylan conversion after 24 h hydrolysis for two different pretreated CS samples at three different protein loadings, while keeping βG loading at 10% supplementation in all experiments. Average (avg.) and standard deviation (s.d.) were based on experiments that were done in triplicate.

no. purified enzymes
xylan conversion for EA-CS(−)
xylan conversion for EA-CS(+)
7.5 mg g−1
15 mg g−1
30 mg g−1
7.5 mg g−1
15 mg g−1
30 mg g−1
CBH I CBH II EG I EX βX avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%) avg. (%) s.d. (%)
1 1 0 0 0 0 2.3 0.1 3.6 0.1 5.8 0.3 2.1 0.0 3.3 0.0 5.3 0.1
2 0 1 0 0 0 1.3 0.1 1.5 0.2 2.1 0.2 1.5 0.2 1.7 0.1 2.2 0.2
3 0 0 1 0 0 6.9 0.5 10.7 0.2 14.2 0.4 6.6 0.2 9.8 0.1 13.1 0.1
4 0 0 0 1 0 13.4 0.7 19.3 0.7 24.2 0.6 12.5 0.4 17.2 1.1 22.5 1.5
5 0 0 0 0 1 2.4 0.2 2.8 0.4 3.3 0.1 2.4 0.1 2.9 0.2 3.2 0.1
6 0.5 0.5 0 0 0 1.8 0.2 2.9 0.1 4.3 0.3 2.0 0.1 2.9 0.1 4.4 0.2
7 0.5 0 0.5 0 0 5.5 0.1 8.3 0.2 11.0 0.3 5.1 0.2 7.6 0.3 10.3 0.2
8 0.5 0 0 0.5 0 10.6 0.4 14.6 0.2 18.1 0.5 8.9 0.2 13.3 0.2 16.2 0.4
9 0.5 0 0 0 0.5 5.5 0.2 8.3 0.1 12.3 0.1 5.1 0.2 7.3 0.1 11.1 0.2
10 0 0.5 0.5 0 0 5.6 0.2 8.3 0.1 11.3 0.2 5.2 0.2 7.2 0.3 10.5 0.3
11 0 0.5 0 0.5 0 10.7 0.3 15.0 0.1 18.3 0.2 9.7 0.5 12.8 0.6 16.8 0.7
12 0 0.5 0 0 0.5 2.3 0.1 3.3 0.2 4.1 0.1 2.6 0.2 3.3 0.5 4.0 0.1
13 0 0 0.5 0.5 0 11.1 0.1 15.4 0.2 19.3 0.4 9.8 0.8 14.1 1.6 19.1 0.2
14 0 0 0.5 0 0.5 29.3 0.2 33.7 0.3 39.5 0.3 24.7 0.6 29.7 0.5 34.7 0.6
15 0 0 0 0.5 0.5 40.3 0.7 45.0 0.9 48.6 0.7 36.6 3.4 40.9 0.5 45.0 2.6
16 0.33 0.33 0.33 0 0 4.8 0.1 7.2 0.1 10.1 0.1 4.3 0.1 6.3 0.2 9.3 0.1
17 0.33 0.33 0 0.33 0 8.5 0.2 11.8 0.2 15.0 0.2 7.3 0.2 11.1 0.9 12.7 0.1
18 0.33 0.33 0 0 0.33 4.8 0.1 7.4 0.1 11.5 0.2 4.6 0.1 6.8 0.1 10.2 0.1
19 0.33 0 0.33 0.33 0 8.6 0.4 11.7 0.5 15.2 0.4 8.1 0.2 11.4 0.5 13.2 0.6
20 0.33 0 0.33 0 0.33 28.4 0.0 34.3 0.2 42.3 1.1 24.3 0.5 30.2 0.6 35.3 1.0
21 0.33 0 0 0.33 0.33 39.2 0.8 44.8 0.3 50.6 2.1 34.0 0.8 41.0 0.4 44.1 3.8
22 0 0.33 0.33 0.33 0 8.9 0.2 12.9 0.5 15.5 0.4 8.6 0.2 12.2 0.2 14.6 0.9
23 0 0.33 0.33 0 0.33 27.2 0.1 33.0 0.4 39.8 0.7 22.9 0.9 28.6 0.5 35.5 1.1
24 0 0.33 0 0.33 0.33 38.2 0.3 43.9 0.2 47.6 0.9 32.5 0.4 40.4 1.8 44.0 3.3
25 0 0 0.33 0.33 0.33 39.2 1.4 43.3 1.1 46.1 1.0 35.1 2.7 37.8 2.8 40.5 0.9
26 0.25 0.25 0.25 0.25 0 7.9 0.1 11.0 0.1 14.1 0.1 7.2 0.1 9.8 0.2 12.3 0.2
27 0.25 0.25 0.25 0 0.25 28.9 0.7 35.2 0.3 42.6 0.4 24.0 0.9 30.3 0.5 35.8 1.0
28 0.25 0.25 0 0.25 0.25 39.2 0.8 44.5 0.6 48.8 1.1 36.3 1.7 38.7 1.1 42.3 0.9
29 0.25 0 0.25 0.25 0.25 41.2 0.3 45.3 0.3 49.9 1.0 40.6 3.8 42.8 0.8 46.6 1.5
30 0 0.25 0.25 0.25 0.25 39.9 0.2 43.9 0.7 49.3 0.9 36.7 1.2 40.9 1.6 47.0 2.5
31 0.2 0.2 0.2 0.2 0.2 39.5 0.1 45.3 0.6 49.2 1.0 37.5 1.1 43.0 1.4 47.9 2.1
32 Ctec2/Htec2 7/3 59.1 0.6 65.1 0.5 71.6 0.6 49.5 0.8 58.8 1.4 66.9 4.5

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