R. Soc. open sci. 5, 171529. (Published 27 June 2018). (doi:10.1098/rsos.171529)
This correction refers to tables 2 and 3; the data of ‘EX’ and ‘βX’ (column; from nos 8 to 31) in these tables have been revised.
Table 2.
Glucan conversion after 24 h hydrolysis for two different pretreated CS samples at three different protein loadings, while keeping βG loading at 10% supplementation in all experiments. Average (avg.) and standard deviation (s.d.) were based on experiments that were done in triplicate.
| no. | purified enzymes |
glucan conversion for EA-CS(−) |
glucan conversion for EA-CS(+) |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7.5 mg g−1 |
15 mg g−1 |
30 mg g−1 |
7.5 mg g−1 |
15 mg g−1 |
30 mg g−1 |
||||||||||||
| CBH I | CBH II | EG I | EX | βX | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | |
| 1 | 1 | 0 | 0 | 0 | 0 | 5.6 | 0.1 | 9.7 | 0.3 | 18.9 | 0.4 | 5.0 | 0.1 | 8.9 | 0.0 | 18.3 | 0.1 |
| 2 | 0 | 1 | 0 | 0 | 0 | 5.8 | 0.1 | 7.5 | 0.1 | 10.0 | 0.2 | 5.1 | 0.2 | 6.2 | 0.1 | 8.2 | 0.0 |
| 3 | 0 | 0 | 1 | 0 | 0 | 16.8 | 0.2 | 20.8 | 0.3 | 25.8 | 0.3 | 13.4 | 0.2 | 16.5 | 0.1 | 21.7 | 0.0 |
| 4 | 0 | 0 | 0 | 1 | 0 | 4.5 | 0.2 | 6.0 | 0.2 | 7.6 | 0.1 | 4.6 | 0.1 | 5.7 | 0.3 | 7.0 | 0.2 |
| 5 | 0 | 0 | 0 | 0 | 1 | 1.7 | 0.2 | 2.2 | 0.3 | 2.5 | 0.1 | 2.2 | 0.1 | 2.6 | 0.0 | 3.0 | 0.1 |
| 6 | 0.5 | 0.5 | 0 | 0 | 0 | 8.4 | 0.2 | 14.1 | 0.2 | 24.6 | 0.2 | 7.1 | 0.1 | 12.1 | 0.1 | 22.5 | 0.2 |
| 7 | 0.5 | 0 | 0.5 | 0 | 0 | 37.2 | 0.2 | 54.6 | 0.0 | 73.9 | 0.2 | 35.8 | 0.4 | 53.2 | 0.5 | 69.1 | 2.5 |
| 8 | 0.5 | 0 | 0 | 0.5 | 0 | 11.5 | 0.2 | 22.0 | 0.4 | 40.5 | 0.3 | 10.9 | 0.0 | 22.1 | 0.2 | 41.0 | 0.2 |
| 9 | 0.5 | 0 | 0 | 0 | 0.5 | 3.7 | 0.1 | 6.2 | 0.2 | 11.9 | 0.1 | 3.8 | 0.2 | 5.7 | 0.1 | 11.2 | 0.2 |
| 10 | 0 | 0.5 | 0.5 | 0 | 0 | 31.0 | 0.4 | 42.9 | 0.2 | 58.7 | 0.4 | 27.7 | 0.5 | 39.0 | 0.7 | 56.7 | 0.8 |
| 11 | 0 | 0.5 | 0 | 0.5 | 0 | 13.3 | 0.1 | 19.9 | 0.2 | 29.8 | 0.3 | 11.9 | 0.3 | 17.7 | 0.0 | 28.9 | 0.3 |
| 12 | 0 | 0.5 | 0 | 0 | 0.5 | 4.7 | 0.2 | 6.3 | 0.1 | 8.2 | 0.2 | 4.6 | 0.3 | 5.4 | 0.2 | 7.0 | 0.1 |
| 13 | 0 | 0 | 0.5 | 0.5 | 0 | 16.2 | 0.1 | 19.3 | 0.0 | 23.4 | 0.2 | 12.8 | 0.4 | 15.4 | 0.7 | 19.5 | 0.2 |
| 14 | 0 | 0 | 0.5 | 0 | 0.5 | 14.6 | 0.2 | 17.9 | 0.1 | 22.2 | 0.1 | 11.5 | 0.3 | 14.0 | 0.2 | 17.7 | 0.1 |
| 15 | 0 | 0 | 0 | 0.5 | 0.5 | 3.4 | 0.1 | 5.1 | 0.2 | 6.5 | 0.0 | 3.8 | 0.2 | 4.9 | 0.0 | 6.3 | 0.2 |
| 16 | 0.33 | 0.33 | 0.33 | 0 | 0 | 52.3 | 0.3 | 69.4 | 0.3 | 82.4 | 1.7 | 50.6 | 0.2 | 65.8 | 4.0 | 77.8 | 4.8 |
| 17 | 0.33 | 0.33 | 0 | 0.33 | 0 | 26.1 | 0.1 | 45.9 | 0.2 | 71.4 | 0.9 | 25.0 | 0.4 | 45.7 | 0.8 | 64.0 | 0.4 |
| 18 | 0.33 | 0.33 | 0 | 0 | 0.33 | 7.0 | 0.2 | 11.5 | 0.1 | 20.0 | 0.2 | 5.9 | 0.0 | 9.5 | 0.2 | 17.8 | 0.2 |
| 19 | 0.33 | 0 | 0.33 | 0.33 | 0 | 41.5 | 0.3 | 58.9 | 0.1 | 79.4 | 1.4 | 38.9 | 0.2 | 56.4 | 1.4 | 71.1 | 3.4 |
| 20 | 0.33 | 0 | 0.33 | 0 | 0.33 | 33.6 | 0.1 | 49.7 | 0.5 | 71.2 | 1.7 | 31.7 | 0.3 | 48.0 | 0.5 | 63.4 | 1.8 |
| 21 | 0.33 | 0 | 0 | 0.33 | 0.33 | 8.8 | 0.2 | 16.3 | 0.2 | 30.7 | 0.0 | 8.7 | 0.1 | 16.1 | 0.1 | 30.7 | 0.5 |
| 22 | 0 | 0.33 | 0.33 | 0.33 | 0 | 31.9 | 0.1 | 43.6 | 0.2 | 58.8 | 0.5 | 29.2 | 0.2 | 40.0 | 0.5 | 56.1 | 1.3 |
| 23 | 0 | 0.33 | 0.33 | 0 | 0.33 | 27.1 | 0.2 | 37.7 | 0.1 | 53.0 | 0.5 | 23.7 | 0.5 | 33.9 | 0.4 | 49.3 | 0.5 |
| 24 | 0 | 0.33 | 0 | 0.33 | 0.33 | 11.0 | 0.2 | 16.5 | 0.3 | 24.7 | 0.2 | 9.5 | 0.2 | 15.1 | 0.0 | 23.3 | 0.3 |
| 25 | 0 | 0 | 0.33 | 0.33 | 0.33 | 14.2 | 0.4 | 17.3 | 0.3 | 21.0 | 0.2 | 11.2 | 0.5 | 13.2 | 0.3 | 16.6 | 0.4 |
| 26 | 0.25 | 0.25 | 0.25 | 0.25 | 0 | 60.8 | 0.3 | 78.0 | 0.4 | 93.9 | 1.5 | 58.8 | 0.4 | 74.4 | 2.2 | 80.4 | 2.5 |
| 27 | 0.25 | 0.25 | 0.25 | 0 | 0.25 | 49.8 | 1.2 | 68.7 | 0.8 | 84.9 | 0.8 | 47.0 | 1.3 | 63.2 | 0.5 | 73.9 | 1.8 |
| 28 | 0.25 | 0.25 | 0 | 0.25 | 0.25 | 20.9 | 0.2 | 38.4 | 0.3 | 63.7 | 1.1 | 20.3 | 0.5 | 38.4 | 0.1 | 61.5 | 0.8 |
| 29 | 0.25 | 0 | 0.25 | 0.25 | 0.25 | 36.5 | 0.3 | 53.0 | 0.1 | 73.8 | 0.6 | 35.3 | 1.4 | 52.0 | 0.0 | 72.5 | 1.3 |
| 30 | 0 | 0.25 | 0.25 | 0.25 | 0.25 | 28.2 | 0.3 | 39.0 | 0.4 | 53.9 | 0.6 | 25.6 | 0.4 | 36.2 | 0.9 | 51.9 | 1.4 |
| 31 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 55.0 | 0.5 | 75.7 | 0.5 | 89.8 | 1.4 | 53.9 | 0.7 | 74.1 | 1.9 | 89.5 | 3.9 |
| 32 | Ctec2/Htec2 7/3 | 73.5 | 0.1 | 88.5 | 0.7 | 99.5 | 1.4 | 65.5 | 1.3 | 81.0 | 0.7 | 94.1 | 1.9 | ||||
Table 3.
Xylan conversion after 24 h hydrolysis for two different pretreated CS samples at three different protein loadings, while keeping βG loading at 10% supplementation in all experiments. Average (avg.) and standard deviation (s.d.) were based on experiments that were done in triplicate.
| no. | purified enzymes |
xylan conversion for EA-CS(−) |
xylan conversion for EA-CS(+) |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7.5 mg g−1 |
15 mg g−1 |
30 mg g−1 |
7.5 mg g−1 |
15 mg g−1 |
30 mg g−1 |
||||||||||||
| CBH I | CBH II | EG I | EX | βX | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | avg. (%) | s.d. (%) | |
| 1 | 1 | 0 | 0 | 0 | 0 | 2.3 | 0.1 | 3.6 | 0.1 | 5.8 | 0.3 | 2.1 | 0.0 | 3.3 | 0.0 | 5.3 | 0.1 |
| 2 | 0 | 1 | 0 | 0 | 0 | 1.3 | 0.1 | 1.5 | 0.2 | 2.1 | 0.2 | 1.5 | 0.2 | 1.7 | 0.1 | 2.2 | 0.2 |
| 3 | 0 | 0 | 1 | 0 | 0 | 6.9 | 0.5 | 10.7 | 0.2 | 14.2 | 0.4 | 6.6 | 0.2 | 9.8 | 0.1 | 13.1 | 0.1 |
| 4 | 0 | 0 | 0 | 1 | 0 | 13.4 | 0.7 | 19.3 | 0.7 | 24.2 | 0.6 | 12.5 | 0.4 | 17.2 | 1.1 | 22.5 | 1.5 |
| 5 | 0 | 0 | 0 | 0 | 1 | 2.4 | 0.2 | 2.8 | 0.4 | 3.3 | 0.1 | 2.4 | 0.1 | 2.9 | 0.2 | 3.2 | 0.1 |
| 6 | 0.5 | 0.5 | 0 | 0 | 0 | 1.8 | 0.2 | 2.9 | 0.1 | 4.3 | 0.3 | 2.0 | 0.1 | 2.9 | 0.1 | 4.4 | 0.2 |
| 7 | 0.5 | 0 | 0.5 | 0 | 0 | 5.5 | 0.1 | 8.3 | 0.2 | 11.0 | 0.3 | 5.1 | 0.2 | 7.6 | 0.3 | 10.3 | 0.2 |
| 8 | 0.5 | 0 | 0 | 0.5 | 0 | 10.6 | 0.4 | 14.6 | 0.2 | 18.1 | 0.5 | 8.9 | 0.2 | 13.3 | 0.2 | 16.2 | 0.4 |
| 9 | 0.5 | 0 | 0 | 0 | 0.5 | 5.5 | 0.2 | 8.3 | 0.1 | 12.3 | 0.1 | 5.1 | 0.2 | 7.3 | 0.1 | 11.1 | 0.2 |
| 10 | 0 | 0.5 | 0.5 | 0 | 0 | 5.6 | 0.2 | 8.3 | 0.1 | 11.3 | 0.2 | 5.2 | 0.2 | 7.2 | 0.3 | 10.5 | 0.3 |
| 11 | 0 | 0.5 | 0 | 0.5 | 0 | 10.7 | 0.3 | 15.0 | 0.1 | 18.3 | 0.2 | 9.7 | 0.5 | 12.8 | 0.6 | 16.8 | 0.7 |
| 12 | 0 | 0.5 | 0 | 0 | 0.5 | 2.3 | 0.1 | 3.3 | 0.2 | 4.1 | 0.1 | 2.6 | 0.2 | 3.3 | 0.5 | 4.0 | 0.1 |
| 13 | 0 | 0 | 0.5 | 0.5 | 0 | 11.1 | 0.1 | 15.4 | 0.2 | 19.3 | 0.4 | 9.8 | 0.8 | 14.1 | 1.6 | 19.1 | 0.2 |
| 14 | 0 | 0 | 0.5 | 0 | 0.5 | 29.3 | 0.2 | 33.7 | 0.3 | 39.5 | 0.3 | 24.7 | 0.6 | 29.7 | 0.5 | 34.7 | 0.6 |
| 15 | 0 | 0 | 0 | 0.5 | 0.5 | 40.3 | 0.7 | 45.0 | 0.9 | 48.6 | 0.7 | 36.6 | 3.4 | 40.9 | 0.5 | 45.0 | 2.6 |
| 16 | 0.33 | 0.33 | 0.33 | 0 | 0 | 4.8 | 0.1 | 7.2 | 0.1 | 10.1 | 0.1 | 4.3 | 0.1 | 6.3 | 0.2 | 9.3 | 0.1 |
| 17 | 0.33 | 0.33 | 0 | 0.33 | 0 | 8.5 | 0.2 | 11.8 | 0.2 | 15.0 | 0.2 | 7.3 | 0.2 | 11.1 | 0.9 | 12.7 | 0.1 |
| 18 | 0.33 | 0.33 | 0 | 0 | 0.33 | 4.8 | 0.1 | 7.4 | 0.1 | 11.5 | 0.2 | 4.6 | 0.1 | 6.8 | 0.1 | 10.2 | 0.1 |
| 19 | 0.33 | 0 | 0.33 | 0.33 | 0 | 8.6 | 0.4 | 11.7 | 0.5 | 15.2 | 0.4 | 8.1 | 0.2 | 11.4 | 0.5 | 13.2 | 0.6 |
| 20 | 0.33 | 0 | 0.33 | 0 | 0.33 | 28.4 | 0.0 | 34.3 | 0.2 | 42.3 | 1.1 | 24.3 | 0.5 | 30.2 | 0.6 | 35.3 | 1.0 |
| 21 | 0.33 | 0 | 0 | 0.33 | 0.33 | 39.2 | 0.8 | 44.8 | 0.3 | 50.6 | 2.1 | 34.0 | 0.8 | 41.0 | 0.4 | 44.1 | 3.8 |
| 22 | 0 | 0.33 | 0.33 | 0.33 | 0 | 8.9 | 0.2 | 12.9 | 0.5 | 15.5 | 0.4 | 8.6 | 0.2 | 12.2 | 0.2 | 14.6 | 0.9 |
| 23 | 0 | 0.33 | 0.33 | 0 | 0.33 | 27.2 | 0.1 | 33.0 | 0.4 | 39.8 | 0.7 | 22.9 | 0.9 | 28.6 | 0.5 | 35.5 | 1.1 |
| 24 | 0 | 0.33 | 0 | 0.33 | 0.33 | 38.2 | 0.3 | 43.9 | 0.2 | 47.6 | 0.9 | 32.5 | 0.4 | 40.4 | 1.8 | 44.0 | 3.3 |
| 25 | 0 | 0 | 0.33 | 0.33 | 0.33 | 39.2 | 1.4 | 43.3 | 1.1 | 46.1 | 1.0 | 35.1 | 2.7 | 37.8 | 2.8 | 40.5 | 0.9 |
| 26 | 0.25 | 0.25 | 0.25 | 0.25 | 0 | 7.9 | 0.1 | 11.0 | 0.1 | 14.1 | 0.1 | 7.2 | 0.1 | 9.8 | 0.2 | 12.3 | 0.2 |
| 27 | 0.25 | 0.25 | 0.25 | 0 | 0.25 | 28.9 | 0.7 | 35.2 | 0.3 | 42.6 | 0.4 | 24.0 | 0.9 | 30.3 | 0.5 | 35.8 | 1.0 |
| 28 | 0.25 | 0.25 | 0 | 0.25 | 0.25 | 39.2 | 0.8 | 44.5 | 0.6 | 48.8 | 1.1 | 36.3 | 1.7 | 38.7 | 1.1 | 42.3 | 0.9 |
| 29 | 0.25 | 0 | 0.25 | 0.25 | 0.25 | 41.2 | 0.3 | 45.3 | 0.3 | 49.9 | 1.0 | 40.6 | 3.8 | 42.8 | 0.8 | 46.6 | 1.5 |
| 30 | 0 | 0.25 | 0.25 | 0.25 | 0.25 | 39.9 | 0.2 | 43.9 | 0.7 | 49.3 | 0.9 | 36.7 | 1.2 | 40.9 | 1.6 | 47.0 | 2.5 |
| 31 | 0.2 | 0.2 | 0.2 | 0.2 | 0.2 | 39.5 | 0.1 | 45.3 | 0.6 | 49.2 | 1.0 | 37.5 | 1.1 | 43.0 | 1.4 | 47.9 | 2.1 |
| 32 | Ctec2/Htec2 7/3 | 59.1 | 0.6 | 65.1 | 0.5 | 71.6 | 0.6 | 49.5 | 0.8 | 58.8 | 1.4 | 66.9 | 4.5 | ||||
