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. 2018 Aug 4;138(10):1012–1024. doi: 10.1161/CIRCULATIONAHA.118.033703

Figure 3.

Figure 3.

Increased cardiomyocyte cell fusion events occur with Kit lineage loss of Gata4/6 within heart cells during bone marrow transplant. A, Experimental design for bone marrow transplant experiments examining the impact of Gata4/6 loss in Kit allele–derived bone marrow on cardiac phenotyping. B, Representative FACS plot showing recombination of eGFP+ bone marrow from a Kit allele lineage–traced donor mouse in a bone marrow transplant recipient. C, Representative FACS plot showing effective irradiation and loss of native mTomato+ bone marrow in a recipient mouse. D, Representative eGFP+ mTomato+ fusion-derived cardiomyocyte from a cardiac histological section following bone marrow transplant. E, Percentage of Kit allele–dependent bone marrow–derived eGFP+ cardiomyocytes from the entire heart of the indicated mice. Error bars are SEM, n=6 per group (10 sections per heart). F, Quantification of cardiac CD31+ cells based on flow cytometry from the interstitial fraction in bone marrow transplant recipient hearts from the indicated mice. Error bars are SEM, n=3 per group, P=n.s. G, Experimental design for bone marrow transplantation to examine the impact of endogenous cardiac loss of Gata4/6 in Kit allele–dependent lineages on the apparent rate of new cardiomyocytes and endothelial cells. H, Percentage of wild-type bone marrow–derived mTomato+ cardiomyocytes within the entire heart following bone marrow transplant in the indicated mice. Error bars are SEM, n=6 per group (10 sections per heart), *P<0.05 vs. KitMCM R26eGFP recipient. I, Quantification from cardiac sections of endogenous endothelial cell number from the Kit lineage with 2 months of tamoxifen induction, with and without bone marrow transplantation, in the indicated mice. Error bars are SEM, n=3 per group, P=n.s. for all pairwise comparisons. BM indicates bone marrow; BMT, bone marrow transplantation; eGFP, enhanced green fluorescent protein; FACS, fluorescence-activated cell sorting; n.s., nonspecific; SSC-A, side scatter area; and Tam, tamoxifen.