FIG 4 .

TaxAss performance compared to FreshTrain-only performance. (A and B) Lake Mendota reads represented by blue bars were incorrectly classified as red bars in the FreshTrain-only classification. Rank order of the bars follows the TaxAss classification rank abundances. Only taxa with at least 0.5% relative abundance are included, and at the lineage level, the number of bars displayed is further truncated to 20. (A) TaxAss maintained phylum richness (blue bars) by classifying phyla using SILVA when they are not included in FreshTrain. (B) TaxAss prevented lineage-level inaccuracies from misclassifications and overclassifications (red bars over known taxa) and lineage-level underclassifications (red bar over “unclassified” category). Versions of this figure across all test ecosystems can be found in Fig. S2.