TABLE 2 .
Agreement between BLAST and recalculated percent identities
| BLAST result |
Recalculated percent identity cutoff applieda: |
|||||
|---|---|---|---|---|---|---|
| 95 | 96 | 97 | 98 | 99 | 100 | |
| Hit 1 | 96.8b | 97.8 | 99.0 | 99.68 | 99.90 | 100 |
| Hit 2 | 1.4 | 1.0 | 0.5 | 0.18 | 0.07 | 0 |
| Hit 3 | 0.5 | 0.3 | 0.1 | 0.04 | 0.01 | 0 |
| Hit 4 | 0.7 | 0.4 | 0.1 | 0.03 | 0.01 | 0 |
| Hit 5 | 0.7 | 0.5 | 0.2 | 0.07 | 0.01 | 0 |
a
Calculations were performed only on sequences above the listed recalculated percent identities.
b
For example, 96.8% of BLAST’s first hits also had the highest recalculated percent identity.