Figure 3. Reduced frequencies of T cell progenitors in Fermt3^-/- thymi.

(A) Thymocytes from Fermt3^+/+ and Fermt3^-/- mice were stained for lineage markers (B220, CD19, TER119, NK1.1, CD11b, Gr-1, CD8α, CD3e, TCRβ, TCRγδ and CD11c), CD44 and c-kit to identify DN_1-2 (Lin^neg, c-kit^hi, CD44^hi) and the DN_3-4 (Lin^neg, c-kit^low, CD44^low) populations. (B and C) PB from Fermt3^+/+ and Fermt3^-/- animals (P3) were stained for lineage markers (TER119, B220, CD11b, Gr-1, CD11c, NK1.1, CD4 and CD8α), c-kit and Sca-1 to identify hematopoietic progenitor cells and analysed by flow cytometry. N = 4. Numbers within the representative FACS plots indicate cell percentages. Bars indicate means ± standard errors. **p<0.01; ***p<0.001. See also Figure 3—figure supplement 1.

Figure 3—figure supplement 1. Reduced frequencies of T cell prognitors in Fermt3^-/- thymi.

Thymocytes from Fermt3^+/+ and Fermt3^-/- P3 mice were stained for lineage markers (B220, CD19, TER119, NK1.1, CD11b, Gr-1, CD8α, CD3ε, TCRβ, TCRγδ and CD11c), CD44 and CD25 to identify and quantify DN1 (Lin^neg, CD44^hi, CD25^-), DN2 (Lin^neg, CD44^hi, CD25⁺), DN3 (Lin^neg, CD44^low, CD25⁺) and DN4 (Lin^neg, CD44^low, CD25^-) populations by flow cytometry. Numbers within FACS plots indicate cell percentages. Total number of DN1, DN2, DN3 and DN4 cells were measured by flow cytometry. N = 20. Bars indicate means ± standard errors. *p<0.05; **p<0.01; ***p<0.001.