Fig. 3.

The role of the P2X7 receptor in mobilization of HSPCs. a The ATP level was evaluated in conditioned medium from Gr-1⁺ cells from P2X7^–/– mice stimulated with G-CSF, AMD3100, or G-CSF + probenecid. Results are shown as a percentage of control WT cells. b The chemotactic responsiveness of WT and P2X7^–/–-derived BM-MNCs to SDF-1, S1P, C1P, and ATP gradients evaluated by FACS (left panel) and clonogenic CFU-GM progenitors (right panel). Results are combined from three independent experiments. *p ≤ 0.05. c For mobilization studies, MNCs were isolated from WT and P2X7^–/– mice after 6 days of UDP-glucose mobilization. The numbers of WBCs, SKL (Sca-1⁺/c-kit⁺/Lin⁻) cells, HSCs (Sca-1⁺/CD45⁺/Lin⁻), and CFU-GM clonogenic progenitors were evaluated in PB. Results from two independent experiments are pooled together. *p ≤ 0.05