Sinomenine alleviates dextran sulfate sodium-induced colitis via the Nrf2/NQO-1 signaling pathway

Abstract

Sinomenine (SIN), a pure alkaloid isolated from Sinomenium acutum, has been widely used in arthritis for its anti-inflammatory effect, but little is known about the effect of SIN on human ulcerative colitis (UC). In the present study, the effect and mechanism of SIN was examined in a dextran sulfate sodium (DSS)-induced murine colitis model, which mimics human UC. Oral administration of SIN significantly suppressed the elevated disease activity index and ameliorated colonic histological damage in a DSS-induced colitis model. Tumor necrosis factor-α, interleukin-6 and inducible nitric oxide synthase levels were also reduced as detected by reverse transcription-quantitative polymerase chain reaction. In addition, SIN reversed the decreased colon length and colonic superoxide dismutase activity. Furthermore, western blot analysis revealed that nuclear factor-erythroid 2-related factor 2 (Nrf2) and its downstream genes, heme oxygenase-1 and NADP(H) quinone oxidoreductase 1 (NQO-1), were markedly activated by SIN. The current results indicated that SIN alleviated DSS-induced colitis in mice, which may be due to its antioxidant properties and was at least in part dependent on the Nrf2/NQO-1 signaling pathway. Therefore, SIN may have potential applications as a protective drug for patients with UC.

Introduction

Ulcerative colitis (UC) is a chronic, relapsing and non-specific immunological-mediated disorder of the gastrointestinal tract. Together with Crohn's disease (CD), it is a type of inflammatory bowel disease (IBD) (1). Individuals suffering from UC are at a high risk of developing colitis-associated cancer, which causes up to 15% of IBD-associated cases of mortality (2). The pathogenesis of UC is complex and has not been clearly elucidated at present. However, genetic, environmental and immunological factors are all thought to be contributors (3). It has been identified that unbalanced cytokine secretion leads to intestinal tissue damage and epithelial barrier disruption in patients with UC and experimental models of colitis (4). Oxidative stress is thought to be a key factor in the development of UC as a regulator of oxidant/antioxidant balance (5).

Nuclear erythroid 2-related factor 2 (Nrf2) plays an important role in antioxidant reactions. It can regulate the transcription of several enzymes in detoxification and antioxidant responses (6). In general, Nrf2 is located in the cytosol, bound to Kelch-like ECH-associated protein 1. When exposed to oxidative stress, Nrf2 enters the nucleus and combines with antioxidant responsive element to regulate the expression of Phase II enzymes, including heme oxygenase 1 (HO-1) and NAD(P)H quinine oxidoreductases (NQOs). These enzymes are critical for maintaining optimal cellular functions (7,8).

Sinomenine hydrochloride (SIN, purity >97%) is an active alkaloid originally extracted from the medicinal herb Sinomenium acutum. SIN exhibits anti-inflammatory and immune-regulatory effects (9,10), and possesses notable therapeutic capacity in treating arthritis (11,12). Studies in vivo have indicated that SIN protects against several autoimmune and inflammation-associated diseases (13,14). In addition, SIN is able to attenuate 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis, an animal model that mimics human CD (15,16). However, the therapeutic mechanism remains unclear. In the current study, the therapeutic effects of SIN were investigated in a dextran sulfate sodium (DSS)-induced colitis model, which possesses similar fundamental clinical and histological features to human UC (17).

In the current study, it was identified that SIN alleviated DSS-induced colitis by producing antioxidant and anti-inflammatory effects, partly via activating the Nrf2/NQO-1 pathway.

Materials and methods

Animals

A total of 40 6–8-week-old female C57BL/6 mice (weight, 18–20 g) were purchased from Cavens Laboratory Animal Co., Ltd. (Changzhou, China). The certification number is SCXK (SU) 2011-0003. The mice were fed a standard laboratory diet, had access to sterile water and were housed under controlled conditions (23±2°C, 50±5% humidity and 12 h light/dark cycle). All animal experiments were approved by the Ethics Committee of Changzhou No. 2 People's Hospital (Changzhou, China), following the ARRIVE guidelines (18).

Reagents

SIN (purity >97%) was purchased from Aladdin (Shanghai, China) and salicylazosulfapyridine (SASP) was obtained from Shanghai Sine Tianping Pharmaceutical Co., Ltd. (Shanghai, China). They were both dissolved in 0.9% NaCl solution. DSS (molecular weight, 36,000–50,000 kDa) was purchased from MP Biomedicals (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The reagent for superoxide dismutase (SOD) examination was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

Induction and assessment of DSS-induced murine colitis model

A total of 40 mice were randomized into four equal groups: Control group, DSS model group, DSS + SASP group and DSS + SIN group. In the control group, colitis was not induced. The DSS group mice were treated with 3% DSS in their drinking water for 7 days and then were given normal drinking water for 3 days for recovery (19,20). SASP and SIN were administered by gavage from day 1 to day 10 at doses of 400 and 100 mg/kg per day, respectively. All mice were sacrificed on day 11. During the course of the experiment, body weight, stool consistency and bleeding scores of every mouse were observed to measure the disease activity index (DAI) (21,22). The scoring system for the DAI is presented in Table I. The rate of body weight gain or loss in each mouse was calculated using the following formula: Body weight change (%) = [(Weight change at day X)-(Weight at Day 1)]/(Weight at Day 1) ×100.

Table I.

DAI scoring system.

Score	Weight loss (%)	Stool consistency	Blood in stool
0	None	Normal	Negative
1	1–5%	Normal	Negative
2	6–10%	Loose stool	Hemoccult positive
3	11–15%	Loose stool	Hemoccult positive
4	>15%	Diarrhea	Gross bleeding

The DAI value is calculated as the scores of weight loss, stool consistency and blood in stool divided by three. Normal stools = well-formed stools; loose stools = pasty stools that did not adhere to the anus; diarrhea = liquid stools that adhered to the anus. DAI, disease activity index.

Histological analysis

After the mice were sacrificed, the colons were removed and the colon length of each animal was measured. The dissected colon tissue was washed with cold phosphate-buffered saline (PBS). Two-thirds of the distal colon was stored at −80°C for biochemical examination, and the rest was fixed in 4% paraformaldehyde for 2 h at room temperature for histopathological analysis. The colon sections were then embedded in paraffin, and stained with hematoxylin and eosin for 5 min at room temperature, according to standard protocols (23). Histological scoring was performed as follows: 0, no signs of inflammation; 1, low leukocyte infiltration; 2, moderate leukocyte infiltration; 3, high leukocyte infiltration, moderate fibrosis, high vascular density, thickening of the colon wall, moderate goblet cell loss and focal loss of crypts; 4, transmural infiltrations, massive loss of goblet cell, extensive fibrosis and diffuse loss of crypts.

SOD activity assessment

Colon tissue was homogenized in cold PBS. The activity level of total superoxide dismutase was detected with total superoxide dismutase assay kit (cat. no. A001-1-1) according to the manufacturer's protocol (Nanjing Jiancheng Bioengineering Institute). The values are expressed as U/mg protein.

RNA isolation and reverse transcription-quantitative polymerase chain reaction (RT-qPCR)

Total RNA was extracted from the colon samples using TRIzol^® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). cDNA was synthesized from RNA using a reverse transcriptase kit (Takara Bio, Inc., Otsu, Japan). The reverse transcription conditions were as follows: 37°C for 15 min, 85°C for 5 sec and hold at 4°C. SYBR Green Master mix (Vazyme, Nanjing, China) and an ABI 7500 system (Applied Biosystems; Thermo Fisher Scientific, Inc.) were used to conduct qPCR, in order to analyze gene expression. The cycling conditions were as follows: Initial step at 95°C for 10 min, followed by 40 cycles at 95°C for 15 sec and 60°C for 1 min. The primer sequences used in the present study are presented in Table II. The mRNA expression level for each target gene was normalized to the level of GAPDH. Expression of target genes was analyzed by the 2^−ΔΔCq method (24).

Table II.

Primer sequences for reverse transcription-quantitative polymerase chain reaction.

Gene	Sequences (5′-3′)
TNF-α
F	CATCTTCTCAAAATTCGAGTGAC
R	TGGGAGTAGACAAGGTACAACCC
IL-6
F	GCTGGTGACAACCACGGCCT
R	AGCCTCGACTTGTGAAGTGGT
iNOS
F	CCAACCTGCAGGTCTTCGATG
R	GTCGATGCACAACTGGGTGAAC
HO-1
F	ATGTGGCCCTGGAGGAGGAGA
R	CGCTGCATGGCTGGTGTGTAG
NQO-1
F	GGATTGGACCGAGCTGGAA
R	AATTGCAGTGAAGATGAAGGCAAC
GAPDH
F	AAGGTCGGAGTCAACGGATTT
R	AGATGATGACCCTTTTGGCTC

TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; iNOS, inducible nitric oxide synthase; HO-1, heme oxygenase-1; NQO-1, NADP (H) quinone oxidoreductase 1; GAPDH, glyceraldehyde-phosphate dehydrogenase; F, forward; R, reverse.

Protein extraction and western blot analysis

Following the treatments, cytosolic and nuclear proteins were isolated from colon samples using a Nuclear and Cytoplasmic Protein Extraction kit (Sangon Biotech Co., Ltd., Shanghai, China) according to the manufacturer's protocol. The protein concentration in the supernatants was determined using an Enhanced BCA Protein Assay kit (Beyotime Institute of Biotechnology, Nanjing, China). Extracts were stored at −70°C until further experimentation.

An equal amount of protein (40 µg) was separated with 12% SDS-PAGE. The proteins were then transferred to polyvinylidene difluoride (PVDF) membranes (EMD Millipore, Billerica, MA, USA) using a wet transfer system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Non-fat milk (5%) was used to block the PVDF membranes for 2 h at 37°C. Proteins were detected using specific primary antibodies against Nrf2 (cat. no. 12721T; rabbit monoclonal; Cell Signaling Technology, Inc., Danvers, MA, USA), NQO-1 (cat. no. ab80588; rabbit monoclonal; Abcam, Cambridge, MA, USA), β-actin (cat. no. 479T; rabbit monoclonal; Cell Signaling Technology, Inc.) and Histone H3 (cat. no. 4499T; rabbit monoclonal; Cell Signaling Technology, Inc.) diluted 1:1,000 overnight at 4°C. Then, the membranes were incubated with goat anti-rabbit IgG-horseradish peroxidase conjugated secondary antibodies (cat. no. sc-2004; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) diluted 1:5,000 for 1.5 h at room temperature. All antibodies were diluted in Primary Antibody Dilution Buffer (Beyotime Institute of Biotechnology). Proteins were visualized using enhanced chemiluminescent reagents (Thermo Fisher Scientific, Inc.). The immunoblots were quantified using ImageJ software version 1.8 (National Institutes of Health, Bethesda, MD, USA).

Statistical analysis

All experimental results were expressed as the mean ± standard error of the mean of three independent experiments. Statistical analysis was performed using Student's t-test or one-way analysis of variance with Tukey's post-hoc test with GraphPad Prism 5 (GraphPad Software, Inc., La Jolla, CA, USA). P<0.05 was considered to indicate a statistically significant difference.

Results

SIN restores DSS-induced colitis

Mice treated with 3% DSS developed severe colitis, which led to body weight loss and high DAI score (body weight change, stool consistency and the presence of blood in the stool) in comparison with the control mice. SASP, an aminosalicylate, was used as a positive control. SASP and SIN administration prevented body weight loss and reduced the DAI score compared with the DSS model group mice (Fig. 1A and B). DSS-induced colonic shortening was also improved by SASP and SIN treatment (Fig. 1C and D).

Figure 1.

SIN ameliorates DSS-induced colitis. The (A) percentage change (%) of mouse body weight, (B) disease activity index and (C and D) colon length of mice treated with DSS alone, DSS + SASP or DSS + SIN. The data are presented as mean ± standard error of the mean. *P<0.05, **P<0.01 vs. control group; ^#P<0.05 vs. DSS group. DSS, dextran sulfate sodium; SIN, sinomenine; SASP, salicylazosulfapyridine.

SIN attenuates colitis histological damage

In the control group, the colons of mice exhibited full structure without any obvious damage (Fig. 2A). However, infiltration of inflammatory cells, defection of crypt structure and mucosal ulceration were observed in the colons of DSS model group mice (Fig. 2B). SASP and SIN treatment attenuated this histological damage (Fig. 2C and D). Histological scores indicated that DSS caused histological defects, while SASP and SIN significantly attenuated these pathological changes (Fig. 2E).

Figure 2.

SIN attenuates histological damage in DSS-induced colitis. Hematoxylin and eosin-stained colon sections from (A) control, (B) DSS, (C) DSS + SASP and (D) DSS + SIN treated mice. Magnification, ×20. (E) Summary of the histological scores. Data are presented as mean ± standard error of the mean. **P<0.01 vs. control group; ^#P<0.05 vs. DSS group. DSS, dextran sulfate sodium; SIN, sinomenine; SASP, salicylazosulfapyridine.

SIN increases SOD activity

To evaluate the antioxidant effects of SIN in a DSS-induced colitis model, the activity of SOD was analyzed using the xanthine/xanthine oxidase method. As presented in Fig. 3, the SOD activity of the DSS-induced colitis group was significantly decreased compared with the control group (P<0.05). Conversely, mice treated with SASP and SIN exhibited increased SOD activity compared with the DSS model group.

Figure 3.

SIN reduces colonic SOD activity. The activity of SOD was analyzed using the xanthine/xanthine oxidase method in the colon tissues of control, DSS, DSS + SASP and DSS + SIN treated mice. Data are represented as mean ± SEM. *P<0.05 vs. control group; ^##P<0.01 vs. DSS group. DSS, dextran sulfate sodium; SIN, sinomenine; SASP, salicylazosulfapyridine; SOD, superoxide dismutase.

SIN decreases mRNA levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and inducible nitric oxide synthase (iNOS)

As presented in Fig. 4A and B, the mRNA levels of TNF-α and IL-6 were greatly increased in colonic tissues following treatment with DSS, whereas SIN significantly suppressed this enhanced expression. DSS treatment enhanced iNOS mRNA expression in the colon compared with the control group (P<0.05), while SASP and SIN treatment significantly inhibited the expression of iNOS induced by DSS (Fig. 4C).

Figure 4.

SIN decreases the mRNA expression levels of TNF-α, IL-6 and iNOS. Reverse transcription-quantitative polymerase chain reaction was performed on gut homogenates to detect (A) TNF-α, (B) IL-6 and (C) iNOS. Data are represented as mean ± standard error of the mean. *P<0.05, **P<0.01 vs. control group; ^#P<0.05, ^##P<0.01 vs. DSS group. DSS, dextran sulfate sodium; IL-6, interleukin-6; iNOS, inducible nitric oxide synthase; SIN, sinomenine; SASP, salicylazosulfapyridine; SOD, superoxide dismutase; TNF-α, tumor necrosis factor-α.

SIN induces the Nrf2/NQO-1 pathway in DSS-induced colitis

Nrf2 protein level was evaluated in the DSS-induced colitis model following treatment with SASP or SIN. As presented in Fig. 5A and B, the nuclear translocation of Nrf2 was significantly improved in the SASP and SIN treatment groups compared with the DSS group. Furthermore, two downstream targets of Nrf2 were examined: HO-1 and NQO-1. The results indicated that the protein level of NQO-1 (Fig. 5C and D) and the mRNA level of HO-1 (Fig. 5E) were significantly increased in SIN-treated mice compared with the DSS group.

Figure 5.

SIN induces the Nrf2/NQO-1 pathway in DSS-induced colitis. Western blot analysis of (A and B) nuclear Nrf2 and (C and D) cytoplasmic NQO-1 protein expression. (E) Reverse transcription-quantitative polymerase chain reaction analysis of HO-1 mRNA expression levels. Data are represented as mean ± standard error of the mean. ^#P<0.05, ^##P<0.01 vs. DSS group. DSS, dextran sulfate sodium; HO-1, heme oxygenase-1; NQO-1, NADP (H) quinone oxidoreductase 1; Nrf2, nuclear factor erythroid 2-related factor 2; SIN, sinomenine; SASP, salicylazosulfapyridine.

Discussion

Ulcerative colitis has a serious negative impact on human health, and is associated with colorectal cancer (25). In clinical practice, aminosalicylates, corticosteroids, immunosuppressors and biological agents are used to treat UC symptoms. These drugs are able to regulate the immune and inflammatory response via specific targets; however, they still have certain adverse effects (26). Therefore, it is important to develop an effective drug for UC with few side effects. In the current study, SASP, an aminosalicylate, was used as a positive control. The effect and mechanism of SIN was investigated in the treatment of DSS-induced colitis.

A previous study demonstrated that TNBS-induced colitis is attenuated by SIN at doses of 30, 100 and 200 mg/kg by gastric gavage (15). Yu et al (16) reported that SIN (100 or 200 mg/kg) administered orally in mice with TNBS-induced colitis results in significant improvements. These findings suggest that the dose and treatment of SIN used in the current study were appropriate.

The current results indicated that DSS-induced colon damage could be ameliorated by SIN treatment, according to the evaluation of DAI, colon length and histological analysis. The DAI in the DSS model group mice increased significantly, whereas SIN administration decreased the DAI. Changes in colon length and histological structure were also detected in the DSS model group and the control group, and SIN attenuated these changes.

Oxidative stress is thought to be a key factor in the development of UC (27). The enhancement of reactive oxygen species and free radicals could play a key role in the pathophysiology of UC. It could lead to destruction of cell membrane integrity resulting from DNA damage, protein oxidation and lipid peroxidation, and subsequent mucosal inflammatory infiltration and ulceration formation (28,29). A decrease of SOD activity may lead to redundant superoxide anions, which usually generates other forms of carbon-, nitrogen- and oxygen-centered radicals, and may aggravate the oxidative damage induced by DSS (30). In the current study, SOD activity was reduced in DSS group mice, while SIN treatment markedly improved the SOD activity. This indicated that SIN administration may counteract DSS-induced colon injury via its antioxidant effect.

It was previously reported that activation of excessive iNOS is correlated with gastrointestinal inflammation, which could accelerate the development of IBD (31). Mouzaoui et al (32) identified that aminoguanidine and curcumin could attenuate the colon damage in IBD by inhibiting iNOS formation. The current results demonstrated that iNOS mRNA expression in DSS group mice was increased, while administration of SIN suppressed these levels.

Pro-inflammatory cytokines play a major role in UC development and progression, particularly TNF-α. TNF-α exhibits a pleiotropic effect in colonic mucosa by activating intracellular signaling (33,34). IL-6 is also one of the crucial cytokines in the pathogenesis of IBD (35). Reduced IL-6 could slow down the development of UC and colitis-associated colon cancer (36). In the current study, RT-qPCR was performed to evaluate the mRNA expression of TNF-α and IL-6 in the colons of mice. DSS-induced colitis mice expressed increased levels of TNF-α and IL-6, while SIN treatment decreased the mRNA levels. This indicated that SIN may alleviate DSS-induced colitis by suppressing the expression of TNF-α and IL-6 at the mRNA level.

Nrf2 signaling plays a crucial role in defending against oxidative stress and inflammation reactions, which are both associated with the occurrence of UC (37). HO-1, an Nrf2 target gene, is activated by stimuli that induce cellular stress, and reduces inflammatory cytokine secretion in numerous diseases, including sepsis and LPS-stimulated macrophages (38,39). Furthermore, HO-1 exerts cytoprotective effects by increasing anti-oxidative capacity and inhibiting oxidative stress (40,41). Numerous natural compounds isolated from plants could ameliorate experimental colitis by activating the Nrf2 signaling pathway. Wagner et al (42) identified that pre-treatment of sulforaphane could reduce DSS-induced colitis in mice by activating Nrf2 signaling and subsequently inhibiting inflammatory mediators. The current results indicated that SIN alleviated DSS-induced colitis via Nrf2 signaling. The mRNA levels of Nrf2 target gene HO-1 were elevated in mice treated with SIN compared with colitis mice. Western blotting indicated that SIN administration activated Nrf2 to induce NQO-1 expression.

In conclusion, the present study demonstrated that SIN treatment alleviated DSS-induced colitis by inhibiting proinflammatory mediators in mice. In addition, SIN may activate the Nrf2/NQO-1 signaling pathway to exert its antioxidant effect. The current study provides evidence that maintaining a balance of oxidative status is important for regulating inflammation in colitis, and that SIN is a potential novel drug for treating UC in patients.

Glossary

Abbreviations

UC

ulcerative colitis

DSS

dextran sulfate sodium

SASP

salicylazosulfapyridine

SIN

sinomenine

DAI

disease activity index

SOD

superoxide dismutase

iNOS

inducible nitric oxide synthase

Nrf2

nuclear factor erythroid 2-related factor 2

HO-1

heme oxygenase-1

NQO-1

NADP (H) quinone oxidoreductase 1

Funding

The present study was supported by grants from the National Natural Science Foundation of China (grant no. 81672799), Changzhou Health and Family Planning Commission Project (grant nos. ZD201606, QN201711) and Nanjing Medical University School Fund (grant nos. 2016NJMUZD081, 2017NJMU043).

Availability of data and materials

All data generated and analyzed during the study are available from the corresponding author on reasonable request.

Authors' contributions

YZ and LT designed the study and wrote the manuscript. YZ, HL, JS and LC conducted the experiments and performed the statistical analysis. YZ and CQ supervised the experiments, analyzed the data and revised the manuscript. All authors have read and approved the final manuscript.

Ethical approval and consent for participation

Research involving animals was approved by the Ethics Committee of the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University (Nanjing, China). All animal protocols performed in this study were conducted strictly based on the guidelines of the Jiangsu Committee on Animal Care.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.