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. 2018 Sep 11;8:13601. doi: 10.1038/s41598-018-31703-z

GmGRP-like gene confers Al tolerance in Arabidopsis

Li Chen 1,2,#, Yupeng Cai 1,2,#, Xiujie Liu 1,2, Chen Guo 1,2, Weiwei Yao 1,2, Shi Sun 2, Cunxiang Wu 2, Bingjun Jiang 2, Tianfu Han 2, Wensheng Hou 1,2,✉
PMCID: PMC6134052  PMID: 30206281

Abstract

Aluminium (Al) toxicity restrains water and nutrient uptake and is toxic to plant roots, ultimately inhibiting crop production. Here, we isolated and characterized a soybean glycine-rich protein-like gene (GmGRPL) that is mainly expressed in the root and that is regulated by Al treatment. Overexpression of GmGRPL can alleviate Al-induced root growth inhibition in Arabidopsis. The levels of IAA and ethylene in GmGRPL-overexpressing hairy roots were lower than those in control and RNA interference-exposed GmGRPL hairy roots with or without Al stress, which were mainly regulated by TAA1 and ACO, respectively. In transgenic soybean hairy roots, the MDA, H2O2 and O2–·content in GmGRPL-overexpressing hairy roots were less than that in control and RNA interference-exposed GmGRPL hairy roots under Al stress. In addition, IAA and ACC can enhance the expression level of the GmGRPL promoter with or without Al stress. These results indicated that GmGRPL can alleviate Al-induced root growth inhibition by regulating the level of IAA and ethylene and improving antioxidant activity.

Introduction

Aluminium (Al) toxicity is a primary factor that reduces crop yields from acidic soils. In acidic soils (pH < 5), the phytotoxic Al3+ ion is dissolved from clay minerals and becomes soluble, producing Al toxicity that restrains water and nutrient uptake and is toxic to plant roots, ultimately inhibiting crop production. Approximately 30% of the world’s arable land and approximately 50% of the world’s potentially arable land are acidic soils1–3. Furthermore, up to 60% of the acidic soils in the world are in developing countries, where food production is critical4,5. Al toxicity has a more prominent impact on crop production and is also exceeded only by drought among the abiotic limitations to crop production.

The most dramatic symptom of Al toxicity is the inhibition of root elongation, which is caused mainly by the inhibition of cell expansibility and division6. As the inhibition of root elongation is observed within 30 min in Al-sensitive cultivar7, it is now generally accepted that Al inhibition of cell expansion is the main cause of the inhibition of root elongation8. Several reports have shown consistently that most Al is bound to the cell wall9,10. For instance, 85–90% of the total Al accumulated by barley roots is tightly bound to cell walls11. Almost 90% of the total Al is associated with the cell walls of cultured tobacco cells9. Removal of Al binding in apoplasts or cell walls by citrate restored root regrowth12. This illustrated that the amount of Al binding in the cell wall is an important factor in Al toxicity. Thus, improving Al tolerance may be achieved by adjusting the Al binding ability to the cell wall or modification of the cell wall components. Recent evidence has shown that the regulation of cell wall component-related genes can influence Al toxicity. Zhu et al. found that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, and has a lower xyloglucan content. This indicated that XTH31 affected Al sensitivity by modulating the cell wall xyloglucan content and Al binding capacity13.

Glycine-rich proteins (GRPs) belong to the family of cell wall structure proteins and exhibit a diversity of structural domains, tissue-specific expression patterns, and functions. Additionally, this diversity led to the concept that GRPs should not be considered as a family of related proteins but as a wide group of proteins that share a common structural domain. It was indicated that GRPs likely participated in multiple physiological processes14. Many lines of evidence have shown that GRPs may improve plant tolerance by improving the stability and reconstruction of the cell wall. Overexpressing AtGRP5 can enhance the elongation of the root and inflorescence axis in Arabidopsis. Thus, AtGRP5 is likely involved in organ growth by promoting cell elongation15. AtGRP9 is involved in lignin synthesis in response to salt stress in Arabidopsis16. Arabidopsis AtGRP2 or AtGRP7 could enhance seed germination and seedling growth under cold stress, and even confer freezing tolerance in Arabidopsis, as well as improve the grain yield of rice under drought stress17–19. A recent study reported that AtGRP3 is implicated in root size and aluminium response pathways in Arabidopsis, and the grp3-1 mutant exhibits longer root and Al tolerance20.

Here, we cloned a soybean glycine-rich protein-like gene (GmGRPL) that contains a glycine-rich protein domain; additionally, it was highly expressed in the root and was induced by Al. The root apex is the major target site of Al toxicity21. Thus, we investigated the role of GmGRPL in Al stress. We found that overexpression of GmGRPL can alleviate Al-induced root growth inhibition in Arabidopsis. In the soybean hairy roots system, we found that GmGRPL enhanced Al resistance by regulating the level of IAA and ethylene and improving the antioxidant activity.

Results

Isolation and bioinformatics analysis of GmGRPL and its promoter structure

The GmGRPL gene is located on chromosome 17, with the gene number Glyma.17G139700. Its genomic sequence only contains one exon. The GmGRPL cDNA is 3, 362 bp in length and contains an open reading frame (ORF) of 513 bp that encodes a protein of 170 aa (Fig. 1a,b). The protein sequence analyses revealed a glycine-rich protein family domain and a hydrophobic seed protein domain in the GmGRPL protein. The glycine-rich protein family domain is located in the N-terminus, and the hydrophobic seed protein domain is found in the C-terminus (Fig. 1c).

Figure 1.

Figure 1

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Diagram of the GmGRPL gene structure and protein domain. (a) GmGRPL gene structure. The GmGRPL cDNA is 3, 362 bp in length and contains an open reading frame (ORF) of 513 bp. (b) GmGRPL protein encodes 170 aa. (c) GmGRPL protein domain. The glycine-rich protein family domain is located in the N-terminus, and the hydrophobic seed protein domain is found in the C-terminus.

A 1, 999-bp promoter fragment upstream of the ATG (start codon) was cloned, and the elements were examined using the PlantCARE web tool. Several putative cis-regulatory elements were deciphered from the promoter sequence of GmGRPL (Table S1). The TATA box sequence elements, which are required for critical and precise transcription initiation, existed in many forms in the promoter region. The CAAT BOX sequences were also found at numerous positions. Some hormone-response elements were present in the promoter region and included the ethylene-responsive element (ERE) and salicylic acid-responsive element (TCA). Some stress-response elements were also present, including heat stress-responsive elements (HSEs), drought stress-responsive elements (MBS), and many light-responsive elements (BOX 4, BOXI, GA, GT1, I-box, TCT).

Expression pattern of GmGRPL

The GmGRPL tissue-specific expression pattern was evaluated by qRT-PCR at different developmental stages in the soybean cultivar ‘Williams 82’. The highest expression level of the GmGRPL transcript was detected in the root, and that in the stem and flower were relatively low; the expression in the leaf and seed were almost undetectable (Fig. 2a).

Figure 2.

Figure 2

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Tissue and Al-inducible expression pattern of GmGRPL by qRT-PCR. The soybean GmActin gene was used as the internal reference gene. (a) Tissue expression pattern of GmGRPL in root, stem, leaf, flower and seed. The different capital letters represent significant differences between tissues by ANOVA (p < 0.01). (b) Al-inducible expression pattern of GmGRPL. The soybean seedlings in the Vc stage (unifoliolate leaf fully developed) were transferred to AlCl3 (20, 50, 100, 150 μM) for 0, 2, 4, 8, 12, 24, 36, 48 h, 3 d, 4 d, 5 d and 6 d. The roots were sampled to analyse the Al-inducible expression pattern. The different capital letters represent significant differences during different times of one treatment by ANOVA (p < 0.01). The error bars represent the SEM. n = 3.

To determine the response of GmGRPL to Al stress, soybean seedlings in the Vc stage (unifoliolate leaf fully developed) subjected to Al treatments were used for qRT-PCR analysis. The expression of GmGRPL in roots was upregulated by Al treatments during 48 h, then it was gradually decreased after 3 days (Fig. 2b).

Overexpression of GmGRPL in Arabidopsis promotes root growth and Al resistance

We investigated GmGRPL function by overexpressing GmGRPL in the Arabidopsis ecotype Col-0. GmGRPL promoted root growth compared with the wild-type plants. The primary roots in GmGRPL-overexpressing plants were longer than those in the wild-type (Fig. 3a,b). To further analyse the responses of root growth under Al stress, the GmGRPL-overexpressing Arabidopsis seedlings were grown on medium treated with different concentrations of AlCl3. Nine days after Al treatment, the root lengths were calculated. Root growth was inhibited in both the wild-type and transgenic lines, but the roots of the GmGRPL-overexpressing lines grew better than those of wild-type plants (Fig. 3a,b). The relative root growth of GmGRPL- overexpressing lines was also higher than that of wild-type plants (Fig. 3c).

Figure 3.

Figure 3

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The root growth of wild type and GmGRPL overexpression Arabidopsis with or without Al treatment. The wild-type and overexpressing GmGRPL Arabidopsis seedlings grown in the 1/2 MS medium containing 0, 4, 8, or 10 µM AlCl3 (pH 4.5) for 9 d. (a) Phenotype of 9-day-old wild-type (WT) and GmGRPL-overexpression seedlings. OX-6 and OX-8 are transgenic lines. Bar, 1 cm. (b) The primary root length was measured. (c) The relative root length growth was calculated. The relative root length growth was calculated using the following formula: the root elongation under Al treatment/the root elongation in Al-free control ×100. The different capital letters represent significant differences between wild-type and GmGRPL overexpression lines by ANOVA (p < 0.01). The error bars represent the SEM. n = 5.

GmGRPL reduced IAA and ethylene level by regulating genes in IAA and ethylene biosynthetic pathway

The indole-3-acetic (IAA) and ethylene are involved in the regulation of root growth. IAA and 1-aminocyclopropane-1-carboxylic acid (ACC) can inhibit root growth with or without Al in Arabidopsis and soybean (Figs S1 and S2). GmGRPL-overexpressing (OX-GmGRPL) hairy roots had lower IAA content (Fig. 4a). With 100 µM AlCl3 treatment, the IAA content was increased in OX-GmGRPL hairy roots, and there was no evident change in the IAA content in control and RNA interference-exposed GmGRPL (RNAi-GmGRPL) hairy roots, but the IAA level in OX-GmGRPL hairy roots was still lower than that in control and RNAi-GmGRPL hairy roots (Fig. 4a). OX-GmGRPL hairy roots also had lower ethylene content (Fig. 4b). With 100 µM AlCl3 treatment, the ethylene content was increased in control, OX-GmGRPL and RNAi-GmGRPL hairy roots. The ethylene level in OX-GmGRPL hairy roots was approximately 83% compared with that in control and 72% compared with that in RNAi-GmGRPL hairy roots (Fig. 4b).

Figure 4.

Figure 4

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Changes in hormones of hairy roots under Al stress and the expression level of genes in its biosynthesis. 0.1 g hairy root tips (10 mm) were used to measure IAA and ethylene content by ELISA Kit. (a) IAA content. (b) Ethylene content. The different capital letters represent significant differences between treatments in control, OX-GmGRPL and RNAi-GmGRPL hairy roots by ANOVA (p < 0.01). (c–f) The expression level of TAA1, NIT, ACS and ACO. qRT-PCR analysis of gene expression levels between control and OX-GmGRPL hairy roots. GmActin was used as an internal reference. The different capital letters represent significant differences between control and OX-GmGRPL hairy roots by ANOVA (p < 0.01). The error bars represent the SEM. n = 3.

Trp aminotransferase (TAA1) and nitrilase (NIT) are two key enzymes in IAA biosynthesis. In OX-GmGRPL hairy roots, the expression of TAA1 was significantly reduced, and the expression of NIT was no obvious change (Fig. 4c,d). The IAA level in OX-GmGRPL hairy roots may be lowered by reducing the TAA1. ACC synthase (ACS) and ACC oxidase (ACO) are two key enzymes in ethylene biosynthesis. In OX-GmGRPL hairy roots, the expression of ACS was decreased slightly and the ACO was significantly reduced (Fig. 4e,f). The ethylene level in OX-GmGRPL hairy roots may be lowered by reducing ACO.

GmGRPL improves antioxidant activity in soybean hairy roots

The transgenic-positive soybean hairy roots were used for Al treatment. The hairy roots induced by K599 strain carrying null vector were used as the control. With Al treatment, the hairy roots were stained with Evans blue and Eriochrome Cyanine R. Evans blue staining is used to detect membrane peroxidation and integrity. Eriochrome Cyanine R staining is an Al-dependent stain that can detect Al accumulation on the surface of the root. The results showed that Evans blue staining was enhanced in all hairy root types under Al stress, with stronger staining in control hairy roots than in OX-GmGRPL hairy roots and weaker staining in control hairy roots than in RNAi-GmGRPL hairy roots. The difference was more obviously observed in hairy root tips (Fig. 5a). OX-GmGRPL hairy roots displayed weaker Eriochrome Cyanine R staining than the control, while RNAi-GmGRPL hairy roots showed stronger staining than those of the control (Fig. 5a). Under Al stress, the Al content was increased in all hairy root types, but it was lower in OX-GmGRPL hairy roots than in control and RNAi-GmGRPL hairy roots (Fig. 5b). These results suggested that GmGRPL overexpression alleviates Al toxicity in the hairy roots.

Figure 5.

Figure 5

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Changes in lipid peroxidation and antioxidant activity of hairy roots under Al stress. The control, OX-GmGRPL and RNAi-GmGRPL hairy roots were treated with or without 100 μM AlCl3 for 24 h. The hairy roots were excised for determination. (a) Evans blue staining and Eriochrome Cyanine R staining. After Al3+ treatment, the hairy roots were washed three times with sterilized water and were stained with Evans blue solution 0.025% (w/v) in 0.5 mM CaCl2 (pH 4.5) and Eriochrome Cyanine R solution 0.01% (w/v) for 10 min. The stained roots were washed in sterilized water until no dye elutes from the roots. The stained roots were photographed. Bar, 1 cm. (b) Al content in hairy roots. 0.5 g hairy roots were extracted to measure Al content using ICP-MS. (c) MDA content. (d) H2O2 content. (e) O2−·content. 0.1 g hairy root tips (10 mm) were homogenized with PBS buffer (pH 7.4), and the supernatant was used for reaction using ELISA Kits. The different capital letters represent significant differences between treatments in control, OX-GmGRPL and RNAi-GmGRPL hairy rootsby ANOVA (p < 0.01). The error bars represent the SEM. n = 3.

Malondialdehyde (MDA) is the final product of lipid peroxidation, which indicates the extent of membrane peroxidation. Al treatment increased the MDA content in the control, OX-GmGRPL and RNAi-GmGRPL hairy roots. The MDA content was higher in control hairy roots than in OX-GmGRPL hairy roots and lower than that in RNAi-GmGRPL hairy roots (Fig. 5c). Similar results were shown with Evans blue staining. These results indicated that less lipid peroxidation was caused by Al stress in OX-GmGRPL hairy roots than in control hairy roots.

Al treatment also induced hydrogen peroxide (H2O2) and super oxygen anion-free radical (O2−) production in hairy roots after 24 h of treatment. The H2O2 content was increased by approximately 1.2-fold at 100 µM AlCl3 compared with that at 0 µM AlCl3 (Fig. 5d). The O2−· content was increased by approximately 1.1-fold at 100 µM AlCl3 compared with that at 0 µM AlCl3 (Fig. 5e). Both the H2O2 and O2−· content in OX-GmGRPL hairy roots was lower than that in control and RNAi-GmGRPL hairy roots.

Histochemical detection of GmGRPL promoter in soybean hairy roots and Arabidopsis

Transgenic ProGmGRPL::GUS Arabidopsis and soybean hairy roots were used for the analysis of the promoter expression pattern. GUS staining in hairy roots showed that the GmGRPL promoter was mainly expressed in hairy root tips (Fig. 6a,b); in some hairy roots, the GmGRPL promoter was detected in both the root tip and stele (Fig. 6c). The paraffin section also showed that the expression of GmGRPL promoter occurred in pericycle cells (Fig. 6d–f). GUS staining in transgenic Arabidopsis showed that the GmGRPL promoter was expressed only in the root tip, and GUS staining was not detected in other organs (Fig. 6g–i).

Figure 6.

Figure 6

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Expression pattern of the GmGRPL promoter. (a–c) GUS staining in soybean hairy roots (a,b) GUS staining only detect in hairy root tips (c) GUS staining detect in both root tips and stele. Bar, 1 cm. (d–f) Paraffin section for GUS-positive hairy roots (d,e) Cross section of GUS-positive hairy roots (f) Longitudinal section of GUS-positive hairy roots. Bar, 100 μm. (g–i) GUS staining detect only in root tips in transgenic Arabidopsis (g) Two-day-old seedling for GUS staining. Bar, 500 μm. (h) 10-day-old seedling for GUS staining. Bar, 1 mm. (i) The root of 20 d seedlings for GUS staining. Bar, 1 cm.

To assess the inducible expression pattern of the GmGRPL promoter, the transgenic ProGmGRPL::GUS Arabidopsis seedlings were treated with hormones and Al stress. We tested the expression pattern in 10-day-old transgenic seedlings with 10 µM AlCl3, 20 µM AlCl3, 1 µM IAA, or 10 µM 1-aminocyclopropane-1-carboxylic acid (ACC) treatment for 24 h. In addition, after 10 µM AlCl3 treatment, the seedlings were also co-treated with 1 µM IAA and 10 µM ACC, respectively, for 24 h. The seedlings treated with H2O were used as the control. After treatment, the seedlings were subjected to GUS staining overnight. GmGRPL promoter activity was upregulated by AlCl3. Single IAA and ACC treatment could enhance the promoter expression. Furthermore, IAA and ACC increased the GmGRPL promoter expression under Al stress (Fig. 7a–c).

Figure 7.

Figure 7

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Inducible expression pattern of the GmGRPL promoter in Arabidopsis. Group represents 30 seedling roots together for GUS staining. Bar, 5 mm. Indivitual represents one root in the group. Bar, 100 μm. (a) GUS staining in transgenic Arabidopsis roots with single treatments of Al, ACC and IAA, and co-treatments of Al, ACC, and IAA. (b) Relative expression level of GUS with single treatments of Al, ACC and IAA in transgenic Arabidopsis roots. (c) Relative expression level of GUS with co-treatments of Al, ACC, and IAA in transgenic Arabidopsis roots. The different capital letters represent significant differences between treatments by ANOVA (p < 0.01). The error bars represent the SEM. n = 20.

Discussion

In this study, we described the novel gene GmGRPL which was located on chromosome 17, with the gene number Glyma.17G139700. The GmGRPL cDNA is 3, 362 bp in length and contains an ORF of 513 bp that encodes a protein with unknown function. The protein contained two protein domains, including a glycine-rich protein family domain and a hydrophobic seed protein domain (Fig. 1). We also obtained 1,999 bp promoter sequence of GmGRPL. Some hormone-related motifs were predicted to be present in the promoter region, including ethylene-responsive element (ERE) and salicylic acid-responsive element (TCA). The GmGRPL contains a typical glycine-rich protein domain which may be belonged to the family of cell wall structure proteins. Recent evidence has shown that the regulation of cell wall component-related genes can influence Al toxicity. In addition, the soybean GmGRPL gene is tissue specific and is highly expressed in the root (Fig. 2a), and the expression level of GmGRPL is regulated by Al (Fig. 2b). These findings suggest that GmGRPL may response to Al toxicity.

At present, several possible mechanisms for Al toxicity have been proposed; among them, organic acid secretion is considered the most important. Improving the secretion of malic acid, oxalic acid and citric acid, as well as other organic acids, can enhance aluminium tolerance22–25. For example, overexpressing TaALMT1 enhanced malic acid secretion and Al resistance. A new interpretation about organic acid secretion has been proposed, indicating that the organic acids are more likely to interact with the cell wall, reducing the sites of Al and Al accumulation in the cell wall. The cell wall may play a more important role in Al resistance26. Yang reported that the ARF double mutant arf10/16 showed greater Al resistance than the wild type. Many cell wall modification-related genes were regulated by ARF10 and ARF16. The results showed that auxin-regulated Al-induced inhibition of root growth arose from auxin signalling-regulated modification of the cell wall structure or components. Cell wall modification is probably a further downstream response to Al and contributes to the auxin-mediated root growth inhibition in response to Al stress27. The upregulation of cell wall-regulated genes in the arf10/16 double mutant primarily encoding cell wall structure proteins, including HRGPs, arabinogalactan proteins, and various Pro- and Gly-rich proteins, suggests that its reduced sensitivity to Al-induced inhibition of root growth is probably the outcome of a complex network, including structure assembly and remodelling. A potential role for HRGP may alter cell wall porosity and, as a result, reduces the mobility of Al in the root apoplast28,29. XTH31 may alleviate Al toxicity by reducing the xyloglucan content of the cell wall, thereby lowering its Al binding sites13. Our study was performed using Agrobacterium rhizogenes and taking advantage of the soybean hairy root system. We can get the OX-hairy roots and RNAi-hairy roots in vitro, but the transgenic plants could not be generated by this system, the root growth test could not be achieved using hairy root system. And the root growth test with Al stress in our study was studied in transgenic Arabidopsis. Overexpressing GmGRPL Arabidopsis can reduce the inhibition of root growth under Al stress. The root length in transgenic Arabidopsis is longer than that in the control under Al stress (Fig. 3a,b). This finding suggests that the root shows less Al toxicity in overexpressing GmGRPL Arabidopsis. However, we found that the GmGRPL and AtGRP3 have a different behaviour for Al stress. The grp3-1 mutant exhibited longer root and Al tolerance20. According to the different structural domains and tissue-specific expression patterns of GRPs, we inferred that these diversities of GRPs may lead to different functions.

Auxin is the key regulator of root development30–33. Al-regulated inhibition of root growth is clearly regulated by auxin34,35. Auxin enhanced the Al-induced inhibition of root growth27. We found that OX-GmGRPL hairy roots showed increased IAA content with Al treatment. The RNAi-GmGRPL hairy roots showed similar levels of IAA as the control with or without Al treatment. However, the IAA level of OX-GmGRPL hairy roots with Al treatment was still lower than that of the control and RNAi-GmGRPL hairy roots (Fig. 4a). In addition, ethylene is involved in the regulation of root growth27,36,37. It has been reported that ethylene is important in Al-induced root growth inhibition in many species, including the common bean, Arabidopsis and wheat22,35,38,39. Ethylene can regulate auxin biosynthesis and basipetal auxin transport towards the elongation zone, activating auxin signalling in the root apex and causing root growth inhibition40. The results showed that, without Al treatment, the ethylene content in OX-GmGRPL hairy roots was lower than that in non-transgenic hairy roots and RNAi-GmGRPL hairy roots. Although ethylene was increased in all hairy roots types under Al treatment, the ethylene level of OX-GmGRPL hairy roots was still less than that of non-transgenic hairy roots and RNAi-GmGRPL hairy roots (Fig. 4b). We found that in OX-GmGRPL hairy roots, the expression of TAA1 was significantly reduced, and the expression of NIT was no obvious change (Fig. 4c,d), and the expression of ACS was decreased a little and the ACO was significantly reduced (Fig. 4e,f). These results indicated that GmGRPL could reduce the IAA and ethylene levels by regulating TAA1and ACO. In addition, the promoter expression of GmGRPL was both upregulated by IAA and ACC (Fig. 7a). We inferred that GmGRPL, IAA and ethylene may interact on each other under Al stress.

Further study showed that the Al content is lower in OX-GmGRPL hairy roots than in the control under Al stress (Fig. 5b). The OX-GmGRPL hairy roots have higher ability of oxidative resistance than the control (Fig. 5c–e). Reactive oxygen species (ROS) are important for processes such as growth, development, and responses to biotic and abiotic environments. In plants, the root tips represent a zone of active ROS production41. Previous evidence has indicated that ROS affects the mechanical properties of cells, and an appropriate amount of ROS is essential for cell wall loosening41. However, a high level of ROS will cause cell wall stiffening and inhibition of cell expansion by inducing wall lignification42, as well as damage the integrity of the plasma membrane by causing lipid peroxidation43. We found that the MDA content is higher in control hairy roots than in OX-GmGRPL hairy roost (Fig. 5c). Furthermore, Evans blue staining in control hairy roots is stronger than that in OX-GmGRPL hairy roots (Fig. 5a). These results indicated that Al causes more serious oxidative stress in control hairy roots than in OX-GmGRPL hairy roots. The results showed that the levels of ROS, including H2O2, O2−·, in control hairy roots were higher than those in OX-GmGRPL hairy roots (Fig. 5d,e), suggesting that the Al-induced increase in ROS may be an important cause of Al-induced cell rigidity. Thus, GmGRPL also can enhance the antioxidant ability under Al stress.

In conclusion, this study revealed an important role of GmGRPL in Al resistance in Arabidopsis. The overexpression of GmGRPL was found to alleviate Al toxicity by regulating the level of IAA and ethylene and improving the antioxidant activity.

Methods

Plant materials and growth conditions

Soybean cultivar ‘Williams 82’ was cultivated in a greenhouse under 16 h light/8 h dark cycles at 28 °C. Arabidopsis thaliana ecotype Col-0 was planted at 22 °C under16 h light/8 h dark cycles.

Isolation of GmGRPL and its promoter

Total RNA was isolated from ‘Williams 82’ roots using the TransZol Up Plus RNA Kit (TransGen Biotech). The primers for the amplification of GmGRPL cDNA were GmGRPL-F and GmGRPL-R (Table S2). The PCR product was purified and inserted into the pMD18-T cloning vector (Takara) and then sequenced. Genomic DNA was extracted using the CTAB method from ‘Williams 82’ leaves. According to the ‘Williams 82’ genomic data, an approximately 2-kb promoter sequence upstream from ATG was cloned and sequenced using specific primers (Pro-GmGRPL-F and Pro-GmGRPL-R) (Table S2). The cis-regulatory elements in the promoter were analysed by the PlantCARE database (bioformatics.psb.ugent.be/webtools/plantcare).

Construction of the ProGmGRPL::GUS reporter plasmid and OX-GmGRPL and RNAi-GmGRPL plasmids

The plant expression vector pC13P1 has multiple cloning sites, a GUS reporter gene and no promoter. The pMD18-ProGmGRPL vector and pC13P1 vector were double digested with the restriction enzymes KpnI and PstI. The GmGRPL promoter was ligated into the pC13P1 vector with KpnI and PstI digestion to construct the ProGmGRPL::GUS vector. The selection vector pC(Delt)GUS, which has the hygromycin gene but not the GUS gene, was used for co-transformation. The ProGmGRPL::GUS vector and the selection vector pC(Delt)GUS plasmid were introduced into Agrobacterium tumefaciens GV3101 and Agrobacterium rhizogenes K599 using electroporation, respectively.

For the overexpression construct (OX-GmGRPL), the CDS of GmGRPL was inserted into the pGFPGUS vector with a CaMV 35S promoter, GFP and GUS reporter gene. The plasmid was introduced into Agrobacterium rhizogenes K599 and was used for transformation into soybean hairy roots. The CDS of GmGRPL was also inserted into a SP1300 vector with a super promoter and a hygromycin gene. The plasmid was introduced into Agrobacterium tumefaciens GV3101 and was used for transformation into Arabidopsis. For the RNAi construct (RNAi-GmGRPL), the CDS of GmGRPL was inserted into the vector pGFPGUS in the sense and antisense orientations. The plasmid was introduced into Agrobacterium rhizogenes K599 and was used for transformation into soybean hairy roots.

Arabidopsis transformation and soybean hairy root transformation

The Arabidopsis thaliana ecotype Col-0 was used for transformation. The overexpression vector super promoter::GmGRPL was single transformed, and the ProGmGRPL::GUS vector and selection vector pC(Delt)GUS were co-transformed. The transformation was performed using the floral dip method. The seeds of T3 transgenic Arabidopsis were used in this study.

Five-day-old cotyledonary nodes were used for soybean hairy root production44. The expression vectors were OX-GmGRPL and RNAi-GmGRPL. Hairy roots were detected by GFP fluorescence microscopy (Nikon SMZ1500), and the positive hairy roots were used for Al treatment.

Quantitative real-time PCR (qRT-PCR)

The soybean root, stem, leaf, flower, and seed were used to analyse the tissue expression pattern of GmGRPL by qRT-PCR. The soybean seedlings in the Vc stage (unifoliolate leaf fully developed) were transferred to AlCl3 (20, 50, 100, 150 μM) for 0, 2, 4, 8, 12, 24, 36, 48 h, 3 d, 4 d, 5 d and 6 d. The roots were sampled to analyse the Al-inducible expression pattern by qRT-PCR. GmGRPL-specific primers qPCR-GmGRPL-F and qPCR-GmGRPL-R were used for qRT-PCR. The soybean GmActin gene was used as the internal reference gene (Table S2). The GUS gene was used to analyse the inducible expression pattern of the GmGRPL promoter in transgenic ProGmGRPL::GUS Arabidopsis seedling roots. The Arabidopsis AtActin gene was used as the internal reference gene (Table S2). The TAA1, NIT, ACS and ACO genes were used to analyse the change of IAA and ethylene between control and OX-GmGRPL hairy roots by qRT-PCR (Table S2). qRT-PCR reactions were performed according to the three-step method using SYBR Green I dye and the ABI7500 instrument for qRT-PCR. For qRT-PCR, a total volume of 20 μL was used that contained 10 μL of SYBR Premix Ex Taq (2×), 0.4 μL of dye, 0.4 μL of 10 μM of the upstream or downstream primers, 2 μL of cDNA template, and 6.8 μL of ddH2O. Real-time PCR amplification using the standard three-step procedure for denaturation was performed as follows: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s, and 60 °C for 30 s. The relative expression level was calculated using the comparative 2−ΔΔCt method.

Al3+ treatment and Al content measurement

Al3+ treatment was given in the form of aluminium chloride (AlCl3) prepared in 0.5 mM CaCl2 (pH 4.5). After treatment, the hairy roots were washed three times with deionized water. The hairy roots were cut and collected for Al measurement, respectively. Next, 0.5 g of tissue sample was placed in a tank containing 5 ml of HNO3 and 2 ml of H2O2 for 4 h and was digested in a microwave, followed by dilution to 25 g with water as the sample solution. The Al content was measured using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) (Agilent 7700, USA).

Root growth analysis

The overexpressing GmGRPL Arabidopsis seedlings were grown in the 1/2 MS medium containing 0, 4, 8, or 10 µM AlCl3 (pH 4.5) for 9 d. The wide-type Arabidopsis seedlings were grown in the 1/2 MS medium containing 0, 4, 8, 10 µM AlCl3 (pH 4.5) and 0, 10, 50 nM IAA or ACC for 9 d. The soybean ‘Williams 82’ seeds were grown in B5 medium containing 0, 10, 50, 100 µM AlCl3 (pH 4.5) and 0, 10, 50 nM IAA or ACC for 9 d. The seedlings were photographed and the root length was measured using a centimeter scale.

GUS Staining

GUS staining was used to detect the GmGRPL promoter tissue and inducible expression pattern. Histochemical GUS assay was performed according to Jefferson45. The transgenic Arabidopsis and hairy roots with the ProGmGRPL::GUS vector were used to study the promoter expression pattern. The tissues were placed in GUS staining solution (50 mM sodium phosphate at pH 7.0, 0.5 mM potassium ferrocyanide, 0.5 mM potassium ferricyanide, 0.5 mg/ml 5-bromo-4 chloro-3-indolyl-β-D-glucuronide (X-Gluc), 0.1% Triton X-100 and 20% methanol) and were incubated at 37 °C overnight. After staining, the tissue samples were bleached with 50%, 70% and 90% ethanol for 1 h each and were immersed in 70% ethanol overnight. GUS staining was observed under a Nikon SMZ1500 microscope and was photographed with a Nikon DS-Fil.

Evans blue and Eriochrome Cyanine R staining

Hairy roots were used for Al3+ treatment (0, 100 µM) for 24 h. After Al3+ treatment, the hairy roots were washed three times with sterilized water and were stained with Evans blue solution 0.025% (w/v) in 0.5 mM CaCl2 (pH 4.5) and Eriochrome Cyanine R solution 0.01% (w/v) for 10 min. The stained roots were washed in sterilized water until no dye elutes from the roots. The stained roots were photographed.

Antioxidant activity measurement

Hairy root tip (10 mm) tissue samples (0.1 g each) were homogenized with Phosphate buffer saline (PBS) buffer (pH 7.4) at 8000 rpm and 4 °C for 30 min, and the supernatant was used for reaction. The H2O2 content was measured using an enzyme-linked immunosorbent assay (ELISA) kit (SU-B91025). The O2−·, MDA, IAA and ethylene contents were measured using ELISA kits (SU-B91178, SU-B91059, SU-B91094 and SU-B91090, respectively) according to the kit instructions.

Electronic supplementary material

Supplementary information (459.6KB, pdf)

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31601327), Major Science and Technology Projects of China (2016ZX08010-004), Ministry of Science and Technology of China (2016YFD0100504) and Chinese Academy of Agricultural Science and Technology Innovation Project.

Author Contributions

L.C. and Y.C. performed in gene cloning, expression analysis, vector construction, data analysis, and co-wrote the manuscript; X.L. and C.G. assisted in data analysis; W.Y. assisted in soybean hairy roots transformation; S.S. and C.W. provided soybean cultivars and revised the manuscript; B.J. and T.H. revised the manuscript; W.H. designed the study and revised the manuscript. All authors read and approved the final manuscript.

Data Availability Statement

All data generated or analysed during this study are included in this published article (and its Supplementary Information files).

Competing Interests

The authors declare no competing interests.

Footnotes

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Li Chen and Yupeng Cai contributed equally.

Electronic supplementary material

Supplementary information accompanies this paper at 10.1038/s41598-018-31703-z.

References

  • 1.Kochian LV, Hoekenga OA, Piñeros MA. How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorus efficiency. Annu. Rev. Plant Biol. 2004;55:459–493. doi: 10.1146/annurev.arplant.55.031903.141655. [DOI] [PubMed] [Google Scholar]
  • 2.Ma JF. Syndrome of aluminum toxicity and diversity of aluminum resistance in higher plants. Int. Rev. Cytol. 2007;264:225–252. doi: 10.1016/S0074-7696(07)64005-4. [DOI] [PubMed] [Google Scholar]
  • 3.Kochian LV, Piñeros MA, Liu J, Magalhaes JV. Plant adaptation to acid soils: the molecular basis for crop aluminum resistance. Annu. Rev. Plant Biol. 2015;66:571–598. doi: 10.1146/annurev-arplant-043014-114822. [DOI] [PubMed] [Google Scholar]
  • 4.Kochian LV, Piñeros MA, Hoekenga OA. The physiology, genetics and molecular biology of plant aluminum resistance and toxicity. Plant Soil. 2005;274:175–195. doi: 10.1007/s11104-004-1158-7. [DOI] [Google Scholar]
  • 5.Liu J, Piñeros MA, Kochian LV. The role of aluminum sensing and signaling in plant aluminum resistance. J. Integr. Plant Biol. 2014;56:221–230. doi: 10.1111/jipb.12162. [DOI] [PubMed] [Google Scholar]
  • 6.Ma JF, Chen ZC, Shen RF. Molecular mechanisms of Al tolerance in gramineous plants. Plant Soil. 2014;381:1–12. doi: 10.1007/s11104-014-2073-1. [DOI] [Google Scholar]
  • 7.Llugany M, Poschenrieder C, Barceló J. Monitoring of aluminium-induced inhibition of root elongation in four maize cultivars differing in tolerance to aluminium and proton toxicity. Physiol. Plantarum. 1995;93:265–271. doi: 10.1111/j.1399-3054.1995.tb02227.x. [DOI] [Google Scholar]
  • 8.Ma JF, Shen R, Nagao S, Tanimoto E. Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots. Plant Cell Physiol. 2004;45:583–589. doi: 10.1093/pcp/pch060. [DOI] [PubMed] [Google Scholar]
  • 9.Chang YC, Yamamoto Y, Matsumoto H. Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron. Plant Cell & Environment. 1999;22:1009–1017. doi: 10.1046/j.1365-3040.1999.00467.x. [DOI] [Google Scholar]
  • 10.Taylor GJ, et al. Direct measurement of aluminum uptake and distribution in single cells of Chara corallina. Plant Physiol. 2000;123:987–996. doi: 10.1104/pp.123.3.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Clarkson DT. Interactions between aluminum and phosphorus on root surfaces and cell wall material. Plant Soil. 1967;27:347–356. doi: 10.1007/BF01376328. [DOI] [Google Scholar]
  • 12.Liu Q, Yang JL, He LS, Li YY, Zheng SJ. Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale. Biol. Plantarum. 2008;52:87–92. doi: 10.1007/s10535-008-0014-7. [DOI] [Google Scholar]
  • 13.Zhu XF, et al. XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in Arabidopsis. Plant Cell. 2012;24:4731–4747. doi: 10.1105/tpc.112.106039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Sachetto-Martins G, Franco L, de Oliveira D. Plant glycine-rich proteins: a family or just proteins with a common motif? Biochim. Biophys. Acta. 2000;1492:1–14. doi: 10.1016/S0167-4781(00)00064-6. [DOI] [PubMed] [Google Scholar]
  • 15.Mangeon A, et al. AtGRP5, a vacuole-located glycine-rich protein involved in cell elongation. Planta. 2009;230:253–265. doi: 10.1007/s00425-009-0940-4. [DOI] [PubMed] [Google Scholar]
  • 16.Chen AP, et al. Root and vascular tissue-specific expression of glycine-rich protein AtGRP9 and its interaction with AtCAD5, a cinnamyl alcohol dehydrogenase, In Arabidopsis thaliana. J. Plant Res. 2007;120:337–343. doi: 10.1007/s10265-006-0058-8. [DOI] [PubMed] [Google Scholar]
  • 17.Kim JY, et al. Functional characterization of a glycine-rich RNA-binding protein 2 in Arabidopsis thaliana under abiotic stress conditions. Plant J. 2007;50:439–451. doi: 10.1111/j.1365-313X.2007.03057.x. [DOI] [PubMed] [Google Scholar]
  • 18.Kim JS, et al. Glycine-rich RNA-binding protein 7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana. Plant J. 2008;55:455–466. doi: 10.1111/j.1365-313X.2008.03518.x. [DOI] [PubMed] [Google Scholar]
  • 19.Yang DH, et al. Expression of Arabidopsis glycine-rich RNA-binding protein AtGRP2 or AtGRP7 improves grain yield of rice (Orya sativa) under drought stress conditions. Plant Sci. 2014;214:106–112. doi: 10.1016/j.plantsci.2013.10.006. [DOI] [PubMed] [Google Scholar]
  • 20.Mangeon A, et al. AtGRP3 is implicated in root size and aluminum response pathways in Arabidopsis. PLoS ONE. 2016;11:e0150583. doi: 10.1371/journal.pone.0150583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Ryan PR, Kochian LV. Interaction between aluminum toxicity and calcium uptake at the root apex in near-isogenic lines of wheat (Triticum aestivum L.) differing in aluminum tolerance. Plant Physiol. 1993;102:975–982. doi: 10.1104/pp.102.3.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Tian H, et al. Ethylene negatively regulates aluminium-induced malate efflux from wheat roots and tobacco cells transformed with TaALMT1. J. Exp. Bot. 2014;65:2415–2426. doi: 10.1093/jxb/eru123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Furukawa J, et al. An aluminum-activated citrate transporter in barley. Plant Cell Physiol. 2007;48:1081–1091. doi: 10.1093/pcp/pcm091. [DOI] [PubMed] [Google Scholar]
  • 24.Liu MY, et al. The role of VuMATE1 expression in aluminium-inducible citrate secretion in rice bean (Vigna umbellata) roots. J. Exp. Bot. 2013;64:1795–1804. doi: 10.1093/jxb/ert039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Sivaguru M, Liu JP, Kochian LV. Targeted expression of SbMATE in the root distal transition zone is responsible for sorghum aluminum resistance. Plant J. 2013;76:297–307. doi: 10.1111/tpj.12290. [DOI] [PubMed] [Google Scholar]
  • 26.Zheng SJ. The role of cell wall in plant resistance to nutritional stresses and the underlying physiological and molecular mechanisms. Scienitc Sinica vitae. 2014;44:334–341. doi: 10.1360/052014-49. [DOI] [Google Scholar]
  • 27.Yang ZB, et al. TAA1-regulated local auxin biosynthesis in the root-apex transition zone mediates the aluminum-induced inhibition of root growth in Arabidopsis. Plant Cell. 2014;26:2889–2904. doi: 10.1105/tpc.114.127993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Pan W, et al. Al induced cell wall hydroxyproline-rich glycoprotein accumulation is involved in alleviating Al toxicity in rice. Acta Physiol. Plant. 2011;33:601–608. doi: 10.1007/s11738-010-0684-6. [DOI] [Google Scholar]
  • 29.Yang ZB, Eticha D, Rotter B, Rao IM, Horst WJ. Physiological and molecular analysis of polyethylene glycol-induced reduction of aluminum accumulation in the root tips of common bean (Phaseolus vulgaris) New Phytol. 2011;192:99–113. doi: 10.1111/j.1469-8137.2011.03784.x. [DOI] [PubMed] [Google Scholar]
  • 30.Overvoorde P, Fukaki H, Beeckman T. Auxin control of root development. CSH Perspect. Biol. 2010;2:a001537. doi: 10.1101/cshperspect.a001537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Bielach A, Duclercq J, Marhavý P, Benková E. Genetic approach towards the identification of auxin-cytokinin crosstalk components involved in root development. Philos. T. R. Soc. B. 2012;367:1469–1478. doi: 10.1098/rstb.2011.0233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Jansen L, Roberts I, De Rycke R, Beeckman T. Phloem-associated auxin response maxima determine radial positioning of lateral roots in maize. Philos. T. R. Soc. B. 2012;367:1525–1533. doi: 10.1098/rstb.2011.0239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Lavenus J, et al. Lateral root development in Arabidopsis: Fifty shades of auxin. Trends Plant Sci. 2013;18:450–458. doi: 10.1016/j.tplants.2013.04.006. [DOI] [PubMed] [Google Scholar]
  • 34.Kollmeier M, Felle HH, Horst WJ. Genotypical differences in aluminum resistance of maize are expressed in the distal part of the transition zone. Is reduced basipetal auxin flow involved in inhibition of root elongation by aluminum? Plant Physiol. 2000;122:945–956. doi: 10.1104/pp.122.3.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Sun P, Tian QY, Chen J, Zhang WH. Aluminum-induced inhibition of root elongation in Arabidopsis is mediated by ethylene and auxin. J. Exp. Bot. 2010;61:347–356. doi: 10.1093/jxb/erp306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Růzicka K, et al. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution. Plant Cell. 2007;19:2197–2212. doi: 10.1105/tpc.107.052126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Swarup R, et al. Ethylene upregulated auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation. Plant Cell. 2007;19:2186–2196. doi: 10.1105/tpc.107.052100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Massot N, Nicander B, Barceló J, Poschenrieder C, Tillberg E. A rapid increase in cytokine in levels and enhanced ethylene evolution precede Al3+-induced inhibition of root growth in seedlings (Phaeolus vulgaris L.) Plant Growth Regul. 2002;37:105–112. doi: 10.1023/A:1020511007138. [DOI] [Google Scholar]
  • 39.Eticha D, et al. Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaeolus vulgaris) genotypes. Ann. Bot. 2010;105:1119–1128. doi: 10.1093/aob/mcq049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Stepanova AN, Yun J, Likhacheva AV, Alonso JM. Multilevel interactions between ethylene and auxin in Arabidopsis roots. Plant Cell. 2007;19:2169–2185. doi: 10.1105/tpc.107.052068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Liszkay A, van der Zalm E, Schopfer P. Production of reactive oxygen intermediates (O2−, H2O2, and ·OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 2004;136:3114–3123. doi: 10.1104/pp.104.044784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 42.Voothuluru P, Sharp RE. Apoplastic hydrogen peroxide in the growth zone of the maize primary root under water stress. I. Increased levels are specific to the apical region of growth maintenance. J. Exp. Bot. 2013;64:1223–1233. doi: 10.1093/jxb/ers277. [DOI] [PubMed] [Google Scholar]
  • 43.Yamamoto Y, Kobayashi Y, Matsumoto H. Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol. 2001;125:199–208. doi: 10.1104/pp.125.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Cai Y, et al. CRISPR/Cas9-mediated genome editing in soybean hairy roots. PLoS One. 2015;10:e0136064. doi: 10.1371/journal.pone.0136064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Jefferson RA. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 1987;5:387–405. doi: 10.1007/BF02667740. [DOI] [Google Scholar]

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Supplementary Materials

Supplementary information (459.6KB, pdf)

Data Availability Statement

All data generated or analysed during this study are included in this published article (and its Supplementary Information files).

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