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. 2018 Aug 24;9(66):32624–32641. doi: 10.18632/oncotarget.25976

Figure 1. Determination of the solid support matrix for the RPPA system to detect targets in serum samples.

Figure 1

(A) The print map of RPPA array. (B) RPPA assay of MMP-9 in 6 serum samples on NC membranes. Arrays were printed according to the map. (B) Array 1 to Array 4 indicate 4 parallel experiments performed separately. Incubation without anti-MMP-9 antibody was used as a negative control. (C) RPPA assay of MMP-9 in 6 serum samples on glass slides. Array 1 to Array 7 indicate parallel experiments performed separately. Incubation without anti-MMP-9 antibody was used as a negative control.