Figure 3.

Chronological changes of pSer46-MARCKS in human α-Syn-BAC-Tg/GBA-hetero-KO mice. pSer46-MARCKS and pSer129-α-Syn were costained in human normal α-Syn-BAC-Tg/glucocerebrosidase (GBA)-hetero-KO mice at 1, 6, and 24 months of age (3 male mice at each time point). Images were acquired by Olympus FV1200 IX83 confocal microscopy. All bar graphs show average and SEM. Three mice were used for each group, and quantitative analyses of signal intensities (mean pixel intensities) were performed in 10 visual fields (100 × 100 μm) of each mouse, randomly selected from the brain region. Statistical analyses were performed with two-way ANOVA followed by Student’s t test; *, p < 0.05; **, p < 0.01. The increase in phosphorylation was first detected in olfactory bulb and frontal cortex, followed by the temporal and occipital cortices. Signal intensities of pSer46-MARCKS increased rapidly in the temporal and occipital regions and became most prominent among multiple brain regions. (See also Figs. 3-1, 3-2, and 3-3.)