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. 2018 Aug 21;5(4):ENEURO.0228-18.2018. doi: 10.1523/ENEURO.0228-18.2018

Figure 6.

Figure 6.

Sparse activation of Hcrt and inhibitory neurons after CNO injection in Gad2-IRES-Cre;R26R-EYFP mice. A, Microphotographs showing hM3Dq-mCherry labeling in red (left), Hcrt neurons in green (center), and the merged images (right) in Gad2-IRES-Cre;R26R-EYFP mice transfected with AAV-TetO-hM3Dq-mCherry. White arrowheads denote Hcrt neurons coexpressing hM3Dq-mCherry. Scale bar = 100 µm. B, Microphotographs showing Hcrt immunostaining (left), c-FOS staining (center), and the merged images (right) in AAV-injected Gad2-IRES-Cre;R26R-EYFP mice. White arrowheads denote Hcrt neurons that do not express c-FOS. Scale bar = 50 µm. C, Photomicrographs of DAB immunohistochemistry of transfected neurons in LH (brown) and c-FOS expression (black nuclei) after SAL (upper panel) or CNO (lower panel) injection. D, Photomicrographs of GFP-labeled LH inhibitory neurons (green cells) and c-FOS expression (black nuclei) in LH after SAL injection (upper panel) or CNO injection (lower panel). All hypothalamic cells lateral to the dotted white line were included in the Gad2/c-FOS counts. Scale bar = 50 μm. E, GFP-labeled inhibitory neurons in the nRT (green cells) and c-FOS expression (sparse black nuclei) after SAL injection (upper panel) or CNO injection (lower panel). Scale bar = 100 µm (same scale as C).