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. 2018 Jul 11;7(9):e1470730. doi: 10.1080/2162402X.2018.1470730

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© 2018 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — Analysis of culture supernatant from HEK293T cells by two-dimensional electrophoresis (2-DE). Concentrated HEK293T-SN was analyzed by two-dimensional electrophoresis (2-DE). After blotting, membranes were probed with NKp44Fc (A), NKp46Fc (B), or NKp30Fc (C) followed by HRP-conjugated anti-human IgG mAb. HEK293T-SN-biot was subjected to the same procedure and the membrane was incubated with HRP-conjugated Neutravidin (D). In parallel, HEK293T-SN (600 μg) was separated by 2-DE and proteins were stained with “blue silver” colloidal Coomassie (E). Numbers indicate the spots selected for mass spectrometry analysis; in panels (A), (D), and (E) spot 26 is highlighted with a black circle.