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. 2018 Aug 23;7:e38529. doi: 10.7554/eLife.38529

Figure 1. T-DNA insertion within the MpFRH1 promoter results in elevated steady state levels of MpFRH1 mRNA and defective rhizoid precursor cell differentiation.

(A–L) Phenotype of wild type M. polymorpha and the few rhizoid mutants ST21-1, ST45-2 and ST49-10. (A–D) One day old gemma (scale bar 100 μm), (E–H) four day old gemma (scale bar 1 mm), (I–L) 28 day old gemma (scale bar 5 mm) of wild type (A, E, I) and the few rhizoid mutants ST21-1 (N, F, J), ST49-10 (C, G, K) and ST45-2 (D, H, J). The arrowheads indicate rhizoid precursor cells (in A-D) rhizoids (in E-L) and gemma cups (in I-L). (M) Location and orientation of the T-DNA insertion sites within the MpFRH1 locus. L and R stand for T-DNA left and right border, respectively. (N) qRT-PCR analysis of steady state MpFRH1 mRNA levels in 15 day old gemmae of wild type and the few rhizoid mutants ST21-1, ST45-2 and ST49-10. The MpFRH1 transcript level was normalised against MpAPT1.

Figure 1.

Figure 1—figure supplement 1. The few rhizoids phenotype of mutants ST45-2 and ST49-10 co-segregates with the hygromycin resistance marker on the T-DNA.

Figure 1—figure supplement 1.

Frequencies of hygromycin resistant and hygromycin sensitive plants showing mutant and wild type phenotypes in F1 generation from a cross between ST45-2 and ST49-10 with wild type M. polymorpha. Probability (p) of obtaining the observed numbers of hygromycin sensitive and hygromycin resistant F1 plants with the estimated T-DNA copy number was determined using the Chi-square test.