Figure 4. MpFRH1 functions as miRNA.

One day old gemma (A-F, scale bar 200 μm), four day old gemma (F-L, scale bar 1 mm) and 28 day old gemma (M-R, scale bar 5 mm) of wild type (A, G, M), _proACT:MpFRH1 (B, H, N), _proACT:MpFRH1^miRNA (C, I, O), _proACT:MpFRH1^noATG (D, J, P), _proACT:MpFRH1^PSORF1 (E, K, Q) and _proACT:MpFRH1^PSORF2 (F, L, R). The arrowheads indicate rhizoid precursor cells (in A-F) and rhizoids (in G-R).

Figure 4—figure supplement 1. Frequencies of different phenotypes observed in sporelings transformed with partial or modified versions of the MpFRH1 transcript sequence driven by the strong constitutive rice actin promoter.

Phenotypes of the transformant sporelings were scored four weeks after the initial transformation.