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. 2018 Aug 23;7:e38529. doi: 10.7554/eLife.38529

Figure 5. MpFRH1 miRNA-resistant form of MpRSL1 restores rhizoid and gemma development in the MpFRH1GOF2 mutant background.

Three day old gemmae (A–D) of WT (A), MpRSL1GOF2 (B), MpFRH1GOF2; proEF1a:MpRSL1WT (C) and MpFRH1GOF2; proEF1a:MpRSL1res (D), scale bar 500 μm. Gemma cup of mature WT (E), MpRSL1GOF2 (F), MpFRH1GOF2; proEF1a:MpRSL1WT (G) and and MpFRH1GOF2; proEF1a:MpRSL1res (H), scale bar 500 μm. (I) Percentage of three day old gemmae forming rhizoids, n = 18 for each line.

Figure 5.

Figure 5—figure supplement 1. MpFRH1 miRNA-resistant version of MpRSL1 (MpRSL1res) suppresses the MpFRH1GOF2 few rhizoids and few gemmae phenotype.

Figure 5—figure supplement 1.

(A) Sequence of the MpFRH1 miRNA and its target site on the wild type MpRSL1 mRNA. The cleavage site is indicated in red. (B) Point mutations introduced into the MpFRH1 miRNA target site on MpRSL1 mRNA to create MpFRH1 miRNA resistant version of MpRSL1 (MpRSL1res). Mutated bases are indicated in red. (C) Examples phenotypes observed in MpFRH1GOF2 plants transformed with proEF1a:MpRSL1WT or proEF1a:MpRSL1res. Mature transformant plants, Scale bar 2 mm. (D) Frequencies of each phenotype observed in MpFRH1GOF2 plants transformed with either proEF1a:MpRSL1WT or proEF1a:MpRSL1res.