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KO mice consume less food due to higher leptin level. miR-150 KO mice (n = 10) and WT mice (n = 8) fed 60% HFD for 17 weeks. A) Food intake was measured weekly. B) Circulating leptin concentration was measured after 12 weeks HFD. C) Protein levels of mTOR in EAT was measured by Western blotting. D) Western blotting results were quantified by ImageJ. GAPDH was used for normalization. Data represents mean ± SEM, *p < 0.05, **p <0.01.
