. 2018 Sep 17;6:e5432. doi: 10.7717/peerj.5432

Table 2. Primer sets used in this study and their efficiencies.

Gene	Accession number	Primer sequence (5′–3′)	Efficiency (%)	R²	Reference
GAPDH	DQ404538.1	CACCTCAAGATCGTCAGCAA	100.97	0.9949	Chen et al. (2015)
GAPDH	DQ404538.1	GCCGAAGTGGTCATGGAT	100.97	0.9949	Chen et al. (2015)
B2M	DQ404542.1	GGTGGAGCAATCAGACCTGT	93.32	0.9984	Chen et al. (2015)
B2M	DQ404542.1	GCGTTGGGAGTGAACTCAG	93.32	0.9984	Chen et al. (2015)
IL-2	EU638316	CATGCCCAAGAAGGCTACAGAATTG	91.92	0.999	Sitt et al. (2008)
IL-2	EU638316	GTGAATCTTGTTTCAGATCCCTTTAG	91.92	0.999	Sitt et al. (2008)
IL-4	EU-638315	GGAGCTGCCTGTAGAAGACGTCTTTGC	99.25	0.9982	Sitt et al. (2008)
IL-4	EU-638315	CTTCATTCACAGAACAGGTCATGTTTGCC	99.25	0.9982	Sitt et al. (2008)
IL-10	AB775207	TGCTGGAGGACTTTAAGGGTTA	93.14	0.9986	Segawa et al. (2013)
IL-10	AB775207	ATGAAGATGTCAAACTCACTCATG	93.14	0.9986	Segawa et al. (2013)
IL-12	EU638319	CAGACCAGAGCGATGAGGTCTTG	91.08	0.9999	Sitt et al. (2008)
IL-12	EU638319	GGGCTCTTTCTGGTCCTTTAAGATA	91.08	0.9999	Sitt et al. (2008)
IFN-γ	EU638318	CAGAGCCAAATAGTCTCCTTCTACTTC	92.42	0.9976	Sitt et al. (2008)
IFN-γ	EU638318	CTGGATCTGCAGATCATCTACCGGAATTTG	92.42	0.9976	Sitt et al. (2008)
TNF-α	EU638323	GAGGGAAGAGTTCCCAACTGGCTA	101.48	0.9934	Sitt et al. (2008)
TNF-α	EU638323	CTGAGTACTGAGGTTGGCTACAAC	101.48	0.9934	Sitt et al. (2008)