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. 2005 Feb 28;10(2):475–480. doi: 10.3390/10020475

5-Furan-2yl[1,3,4]oxadiazole-2-thiol, 5-Furan-2yl-4H [1,2,4] triazole-3-thiol and Their Thiol-Thione Tautomerism

M Koparır 1,*, A Çetin 1, A Cansız 1
PMCID: PMC6147599  PMID: 18007318

Abstract

5-Furan-2-yl[1,3,4]oxadiazole-2-thiol (Ia) and 5-furan-2-yl-4H-[1,2,4]- triazole-3-thiol (Ib) were synthesized from furan-2-carboxylic acid hydrazide. Mannich bases and methyl derivatives were then prepared. The structures of the synthesized compounds were confirmed by elemental analyses, IR and 1H-NMR spectra. Their thiol-thione tautomeric equilibrium is described.

Keywords: 1,3,4-Oxadiazoles; 1,2,4-triazoles; Mannich bases; thiol-thione tautomerism

Introduction

Triazoles and their derivatives have been proven to be effective bactericides, pesticides and fungicides [1,2,3] Further, some findings that the 1,2,3-triazole nucleus is associated with diverse pharmacological activities such as analgesic, antiasthematic, diuretic, antihypertensive and antiinflammatory properties have made them important chemotherapeutic agents [4,5,6,7]. Derivatives of 1,3,4-oxadiazole are also known to have a broad spectrum of biological activities [8,9,10]. Acyl hydrazides have been in general use as the starting materials in some 1,2,4-triazole and 1,3,4-oxadiazole syntheses [11,12]. In addition there are some studies on electronic structures and thiol-thione tautomeric equilibrium of heterocyclic thione derivatives [13,14,15].

In the present study 5-furan-2-yl[1,3,4]oxadiazole-2-thiol (Ia) and 5-furan-2-yl-4H[1,2,4]-triazole-3-thiol (Ib) and some of their derivatives were synthesized. Compound Ia was synthesized by the ring closure reaction of furan-2-carboxylic acid hydrazide with carbon disulfide. A series of Mannich bases of 5-furan-2-yl[1,3,4]oxadiazole-2-thiol (IIIa-g) were then synthesized by the reaction of Ia with suitably substituted amines and formaldehyde in ethanol. 5-Furan-2-yl-4H[1,2,4]triazole-3-thiol (Ib) was prepared by the reaction of the appropriate 2-furoyl thiosemicarbazide and potassium hydroxide in ethanol for 3 h under reflux, followed by acidification with acetic acid. The 2-furoyl thiosemicarbazide employed in these reactions was obtained by refluxing the corresponding furan-2-carboxylic acid hydrazide with ammonium thiocynate in presence of aq. hydrochloric acid for 3 h. IIa and IIb were obtained from reaction of Ia and Ib with CH3I in an alkaline medium. These synthetic reactions are summarized in Scheme 1.

Scheme 1.

Scheme 1

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Results and Discussion

The characterization data of compounds Ia and Ib are given in the Experimental section and that of the other compounds synthesized is summarized in Table 1. All the newly synthesized compounds gave satisfactory analyses for the proposed structures, which were confirmed on the basis of their IR and 1H-NMR spectral data. The IR spectra of these compounds showed moderately strong bands around 3100-3360 cm-1, 1600-1650 cm-1 and 1250-1270 cm-1, characteristic of the NH, C=N and C=S groups, respectively. In the 1H-NMR spectra, a characteristic signal due to the –N-CH2-N- protons appeared at 5.00-6.05. The signal due to the NH protons appeared at 5.50-5.52. The signals due to the aromatic protons appeared as multiplets at 6.50-8.40.

Table 1.

Analytical and spectroscopic data for compounds IIa,b and IIIa-g.

Comp. No. R X Yield, % mp, ºC IR spectrum, υ, cm-1 1H NMR spectrum, δ, ppm (J, Hz)
IIa O 45 161 2982 (CH),1636(C=N), 1260(C=S). 6.60-7.60 (m, 3H, furyl), 2.10 (s, 3H, SCH3)
IIb NH 50 132 3130(NH), 2982(CH),
1636(C=N),1260(C=S).		 6.60-7.50 (m, 3H, furyl), 5.70 (1H, NH), 2.10 (s, 3H, SCH3
IIIa graphic file with name molecules-10-00475-i001.jpg O 82 133 2990(CH),1632(C=N)
1270(C=S).		 6.56-7.60 (m, 3H, furyl), 5.0 (s, 2H, N-CH2-N)
3.70-3.50 (m, 4H, CH2-O-CH2), 2.70-2.60 (m, CH2-N-CH2).
IIIb graphic file with name molecules-10-00475-i002.jpg O 60 145 3320(NH),2970(CH),1630(C=N),1268(C=S). 8.24-7.60 (m, 4H, Ar.CH), 6.50-7.60 (m, 3H, furyl), 5.50 (br, 1H, N-CH2-NH), 5.90 (d, J=7 2H, N-CH2-NH), 2.15 (s, 3H, CH3).
IIIc graphic file with name molecules-10-00475-i003.jpg O 62 148 3315(NH),2970(CH),1630(C=N),1268(C=S). 8.40-7.80 (m, 4H, Ar.CH), 6.50-7.60 (m, 3H, furyl), 5.50 (br, 1H, N-CH2-NH), 5.90 (d, J=7 2H, N-CH2-NH), 3.70 (s, 3H, OCH3).
IIId graphic file with name molecules-10-00475-i004.jpg O 50 193 3330(NH), 2982(CH),
1636(C=N),1280(C=S).		 8.20-7.80 (m, 7H, Ar.CH), 6.50-7.60 (m, 3H, furyl), 5.52 (br, 1H, N-CH2-NH), 5.85 (d, J=7 2H, N-CH2-NH).
IIIe graphic file with name molecules-10-00475-i005.jpg O 40 128 3323(NH), 2982(CH),
1636(C=N),1260(C=S).		 8.25-7.90 (m, 5H, Ar.CH), 6.50-7.60 (m, 3H, furyl), 5.52 (br, 2H, -NH), 5.92 (d, J=7 2H, N-CH2-NH), 3.80 (d, J=9 Ar-CH2-NH).
IIIf graphic file with name molecules-10-00475-i006.jpg O 70 133 2982(CH),1636(C=N),1265(C=S). 6.50-7.60 (m, 3H, furyl), 5.80 (s, 2H, N-CH2), 2.10-2.80 (m, 10H, CH2).
IIIg graphic file with name molecules-10-00475-i007.jpg O 35 174 3315(NH), 2982(CH),
1636(C=N),1270(C=S).		 8.40-7.80 (m, 4H, Ar.CH), 6.50-7.60 (m, 3H, furyl), 5.50 (br, 1H, N-CH2-NH), 6.05 (d, J=7 2H, N-CH2-NH).
Comp No. Found, % Formula Calculated,%
C H N S C H N S
IIa 46.15 3.29 15.33 17.58 C7H6N2O2S 46.15 3.32 15.37 17.60
IIb 46.38 3.87 23.11 17.65 C7H7N3OS 46.40 3.89 23.19 17.69
IIIa 49.41 4.89 15.72 11.98 C11H13N3O3S 49.43 4.90 15.72 12.00
IIIb 58.49 4.54 14.60 11.13 C14H13N3O2S 58.52 4.56 14.62 11.16
IIIc 55.45 4.32 13.83 10.55 C14H13N3O3S 55.43 4.32 13.85 10.57
IIId 63.13 3.99 13.00 10.00 C17H13N3O2S 63.14 4.05 12.99 9.92
IIIe 58.48 4.55 14.60 11.13 C14H13N3O2S 58.52 4.56 14.62 11.16
IIIf 54.28 5.68 15.78 12.01 C12H15N3O2S 54.32 5.70 15.84 12.08
IIIg 48.98 3.15 17.59 10.10 C13H10N4O4S 49.05 3.17 17.60 10.07
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We have observed that extensive thiol-thione tautomerism exists in compounds Ia and Ib. In the 1H-NMR the signal of the SH protons were recorded, although they were very weak and also the ready synthesis of the Mannich bases IIa, IIIa-g, IIb and Ib [16] from Ia and Ib confirmed the tautomerism. It has been reported that the crystal structures of Ia- and Ib-like compounds correspond to the thione form [17,18,19], but the reaction conditions for the synthesis of IIa prove that Ia can be in the thiol form too. Finally, the crystal structures of Ia and Ib [17,18] corresponded to the thione form, but they showed thiol-thione tautomerism in solution.

Experimental

General

Melting points were determined in open capillary tubes on a digital Gallenkamp melting point apparatus and are uncorrected. The IR spectra were recorded in KBr with a Mattson 1000 FT-IR spectrometer. 1H-NMR spectra were recorded on a FX 90 JEOL 90MHz NMR and Varian Gemini 200MHz, spectrometers in CDCl3 + DMSO- d6 with TMS as an internal standard. Elemental analyses were done on a LECO-CHNS-938. Starting chemicals were obtained from Merck or Aldrich.

5-Furan-2-yl[1,3,4]oxadiazole-2-thiol (Ia). A mixture of furan-2-carboxylic acid hydrazide (0.01 mole, 1.26 g), sodium hydroxide (0.01 mole, 0.4 g), carbon disulfide (0.02 mole, 1.2 mL) and absolute ethanol (100 mL) was heated under reflux for 12 h. The excess solvent was removed by vacuum evaporation, and the residue was dissolved in water and acidified with acetic acid. The product was recrystallised from water-ethanol (60-40). Yield 55 %; mp: 135-137 oC; IR, cm-1: 3356 (NH), 1642 (C=N), 1255(C=S); 1H-NMR, ppm: 6.56-7.65 (m, 3H, furyl), 13.70 (s, 1H, SH).

5-Furan-2-yl-4H[1,2,4]triazole-3-thiol (Ib). An equimolar quantity of furan-2-carboxylic acid hydrazide (0.01 mole, 1.26 g), ammonium thiocyanate (0.01 mole, 1.52 g) and hydrocholoric acid (5 mL) in absolute ethanol (50 mL) was refluxed for 4 h. The white solid that appeared on cooling was filtered and the excess solvent was removed by vacuum evaporation. The residue was recrystallised from DMF-ethanol (30-70 v/v) to give 1-(2-furoyl)-3-thiosemicarbazide (Yield 90 %; mp: 233-235 oC). This intermediate (0.01 mole 1.85 g) was refluxed in 10 % sodium hydroxide solution (5 mL) for 3 h. The resulting solution was cooled and filtered. The filtrate was acidified with hydrocholoric acid to pH 5-6. The solid which appeared was filtered, dried and recrystallised from dilute ethanol. Yield 75 %; mp: 295 oC; IR, cm-1: 3356-3155 (NH), 1642 (C=N), 1255(C=S); 1H-NMR, ppm: 13.80 (s, 1H, SH), 6.56-7.65 (m, 3H, furyl), 5.10 (s, 1H, NH).

General Procedure for the Preparation of IIa,b.

A mixture of thione Ia-b (0.005 mole), sodium hydroxide (0.005 mole, 0.2 g), and methyl iodide (0.006 mole, 0.840 g) was stirred in water for 14 h. The resulting thioether solution was removed by vacuum evaporation, and the products collected by filtration, washed with water, dried and recrystallised from a suitable solvent. Spectroscopic and physical data are summarized in Table 1.

General Procedure for the Preparation of IIIa-g.

A mixture of Ia (0.01 mole, 1.56 g) and an alkyl or aryl amine (0.01 mole) was refluxed in ethanol (50 mL) with 36 % formaldehyde (0.02 mole, 1.7 mL) for 3 h. The resulting solid was crystallised from a suitable solvent. Spectroscopic and physical data are summarized in Table 1.

Footnotes

Sample availability: Available from the authors.

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