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(A) Immunohistochemical staining of native, CHAPS-treated, SDS-treated and NaOH-treated decellularized lungs for fibronectin, laminin and β-actin. Scale bar = 10 μm. (B) Bar graph showing immune-positive area per alveolus of fibronectin, laminin and β-actin, which was calculated in image J software. Asterisk indicates significance of difference between the groups (*P < 0.005, **P < 0.05).
