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. 2017 Oct 27;22(11):1840. doi: 10.3390/molecules22111840

Figure 2.

Figure 2

Analysis of DNA/PNA complexes by gel mobility shift assay. (A) All lanes contain Cy3-labelled ssDNA (DNA1) at 100 nM. Each lane from the left side contains no PNA, 100 nM of PNA1, 2, 6, 3, 4, and 5, respectively. Each PNA and DNA1 were incubated in 10 mM sodium phosphate buffer and 1 mM EDTA (pH 6.0) at 25 °C for 10 min. Gel mobility shift assays of PNA/DNA complexes were performed using 15% non-denaturing polyacrylamide gels; and (B) quantitative band-intensity analysis of DNA and DNA/PNA complexes in Figure 2.