Table 2.
Gene specific primer sequences used in qPCR assay.
| Gene Symbol | Accession Number 1 | Forward Primer Sequence | Reverse Primer Sequence |
|---|---|---|---|
| TOP2A | NM_001067.3 | 5′-GACGCTTCGTTATGGGAAGATA-3′ | 5′- GGGCCAGTTGTGATGGATAA -3′ |
| MDR1 | NM_001348946.1 | 5′-CAGCTATTCGAAGAGTGGGC-3′ | 5′-CCTGACTCACCACACCAATG-3′ |
| MRP1 | NM_004996.3 | 5′-ACCAAGACGTATCAGGTGGC-3′ | 5′-CTGTCAGGTTCCAGCTCCTC-3′ |
| MRP2 | NM_000392.4 | 5′-GCAGCGATTTCTGAAACACA-3′ | 5′-CAACAGCCACAATGTTGGTC-3′ |
| MRP3 | NM_003786.3 | 5′-CGCACACCGGCTTAACACTATCATGG-3′ | 5′-AAACCAGGAAAGGCCAGGAGGAAATC-3′ |
| MRP4 | NM_001301829.1 | 5′-GAGTTGCAAGGGTTCTGGGA-3′ | 5′-AAAGTCAGCACCGTGGCATA-3′ |
| RPS18 | NM_022551.2 | 5′-GATATGCTCATGTGGTGTTG-3′ | 5′-AATCTTCTTCAGTCGCTCCA-3′ |
| MDR1-promoter | - | 5′-GCAAGCTTCTAGAGAGGTGCAAC-3′ | 5′-AAAAGCTTGCGGCCTCTG-3′ |
| MRP1-promoter | - | 5′-TCTGTGTGACTCAGCTTTGG-3′ | 5′-GTGCAGAGAGGTTGAGTGATT-3′ |
1 Accession number from GeneBank.