Table 1.
Effects of colchicine, N-(Phenylmethyl)-7H-purin-6-amine (N6-benzyladenine, BA), and 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron, TDZ) on plantlet and subsequent polyploidy formation from the nodal-stem explants of Anoectochilus formosanus.
| Colchicine (mg/L) | 0.5 mg/L BA-Containing Medium | 0.5 mg/L TDZ-Containing Medium | ||
|---|---|---|---|---|
| * Number of Survival Shoots | ** Percentage of Polyploids (%) | Number of Survival Shoots | Percentage of Polyploids (%) | |
| 0 (control) | 11.0 ± 1.2a *** | 0 | 14.5 ± 0.6a | 0 |
| 100 | 5.3 ± 0.5b | 10 | 8.3 ± 1.3b | 50 |
| 250 | 2.5 ± 1.0c | 20 | 3.0 ± 1.4c | 20 |
| 500 | 1.5 ± 0.6cd | 20 | 1.8 ± 0.5cd | 20 |
| 1000 | 1.0 ± 0.0d | 10 | 1.3 ± 0.5d | 10 |
Four replicates (each contains five nodal-stem explants and each explant has three nodes) were used in each treatment. * Data were scored after three months of culture; ** Data were scored after one year of culture, 10 plantlets were selected randomly in each treatment to evaluate the polyploidy; *** Means ± Standard Deviation (SD) within a column followed by the same letter are not significantly different according to Duncan’s multiple range test (P ≤ 0.05).