Table 6.

Analytical conditions of high-performance liquid chromatography (HPLC) for analysis of the three standards.

Parameter	Condition
Column	Zorbax extended C18 (C18; 4.6 mm × 150 mm, 5 μm)
Flow rate	0.8 mL/min
Injection volume	10 μL
UV detection	340 nm
Run time	24 min
Gradient	Time (min)	% A 1	% B 2
	0	10	90
	5	10	90
	18	50	50
	20	100	0
	21	10	90
	25	10	90

¹ Acetonitrile; ² 0.2% phosphoric acid.