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. 2017 Sep 8;22(9):1498. doi: 10.3390/molecules22091498

Figure 6.

Figure 6

Hop-8 did not interfere in the interaction of Vif with A3G, CBF-β, Elongin C, or Cullin 5. (A) Hop-8 did not interfere in Vif and A3G interaction. The plasmid pcDNA3.1-APOBEC3G-HA (3 μg) was transfected into 2 × 106 Trex-hvif-15 cells and treated with 0.1 μg/mL Dox and 2.7 μM hop-8 or DMSO for 48 h. Cells were treated with 10 μM MG-132 for 16 h. Co-IP assays were performed with an anti-HIV1 Vif antibody; (B–D) Hop-8 did not block the interaction between Vif and CBF-β (B), Elongin C (C), or Cullin 5 (D). The plasmids pcDNA3.1-Vif-HA (1.5 μg) and pcDNA3.1-CBF-β-FLAG (1.5 μg) or pcDNA3.1-Elogin C-FLAG or pcDNA3.1-Cullin 5-FLAG were co-transfected into 2 × 106 293T cells. Cells were treated with 2.7 μM hop-8 or DMSO. Co-IP assays were performed with an anti-HA antibody; (E) The influence of hop-8 on A3G ubiquitination. The plasmids pcDNA3.1-APOBEC3G-HA (1.5 μg) and pUb-MYC (1.5 μg) were co-transfected into 2 × 106 Trex-hvif-15 cells. Cells were treated with 0.1 μg/mL Dox and 2.7 μM hop-8 or DMSO. The Co-IP assay was performed with a rabbit anti-HA antibody.